SyntheticpeptidewithinHumanCoRESTaa1-100.Theexactsequenceisproprietary.
Databaselink:Q9UKL0

HeLa,Molt-4andJurkatcelllysates;HumancervixcarcinomaandRatcolontissues;HeLacells.

Thisproductisarecombinantmonoclonalantibody,whichoffersseveraladvantagesincluding:-Highbatch-to-batchconsistencyandreproducibility-Improvedsensitivityandspecificity-Long-termsecurityofsupply-Animal-freeproductionFormoreinformationseehere.

OurRaBMAbtechnologyisapatentedhybridoma-basedtechnologyformakingrabbitmonoclonalantibodies.Fordetailsonourpatents,pleaserefertoRabMAbpatents.

存放说明Shippedat4°C.Storeat+4°Cshortterm(1-2weeks).Upondeliveryaliquot.Storeat-20°Clongterm.Avoidfreeze/thawcycle.
存储溶液Preservative:0.01%Sodiumazide
Constituents:59%PBS,40%Glycerol,0.05%BSA

Theapplicationnotesincluderecommendedstartingdilutions;optimaldilutions/concentrationsshouldbedeterminedbytheenduser.

Nuclearreceptorcoregulator1antibodyNuclearreceptorsubfamily2groupBmember2antibodyProteinCoRESTantibodyRCORantibodyRcor1antibodyRCOR1_HUMANantibodyRESTcorepressor1antibodyRetinoicacidreceptorRXR-betaantibodyRetinoidXreceptorbetaantibodyRxrbantibodyseeall
Alllanes:Anti-CoRESTantibody[EPR13825](ab183711)at1/10000dilution

Lane1:HeLacelllysate
Lane2:Molt-4celllysate
Lane3:Jurkatcelllysate

Lysates/proteinsat20µgperlane.

Secondary
Alllanes:GoatAnti-RabbitIgG,(H+L),Peroxidaseconjugateat1/1000dilution

Predictedbandsize:53kDa
Observedbandsize:66kDawhyistheactualbandsizedifferentfromthepredicted?

Immunohistochemicalanalysisofparaffin-embeddedHumancervixcarcinomatissuelabelingCoRESTwithab183711at1/50dilutionfollowedbypredilutedHRPPolymerforRabbitIgG.CounterstainedwithHematoxylin.

PerformheatmediatedantigenretrievalwithEDTAbufferpH9beforecommencingwithIHCstainingprotocol.

Immunohistochemicalanalysisofparaffin-embeddedRatcolontissuelabelingCoRESTwithab183711at1/50dilutionfollowedbypredilutedHRPPolymerforRabbitIgG.CounterstainedwithHematoxylin

PerformheatmediatedantigenretrievalwithEDTAbufferpH9beforecommencingwithIHCstainingprotocol.

Immunofluorescentanalysisofacetone-fixedHeLacellslabelingCoRESTwithab183711at1/100dilution,followedbyGoatantirabbitIgG(AlexaFluor488)secondaryantibodyat1/200dilution(green).CounterstainedwithDapi(blue).

TodownloadaCertificateofCompliance,pleaseenteryourLotnumberbelow:

Lotnumber

发表研究结果有使用ab183711？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab183711被引用在2文献中.

MehtaSLetal.LongNoncodingRNAFosDTPromotesIschemicBrainInjurybyInteractingwithREST-AssociatedChromatin-ModifyingProteins.JNeurosci35:16443-9(2015).PubMed:26674869ChanFCetal.AnRCOR1loss-associatedgeneexpressionsignatureidentifiesaprognosticallysignificantDLBCLsubgroup.Blood125:959-66(2015).HumanPubMed:25395426
SampleRatTissuelysate-nuclear(Hippocampus,liverandMCF7wholecellhpmogenates)
BlockingstepMilkasblockingagentfor1hour(s)and0minute(s)Concentration:5%Temperature:25C

Pleasenote:AllproductsareFORRESEARCHUSEONLY.NOTFORUSEINDIAGNOSTICPROCEDURES
Forlicensinginquiries,pleasecontactpartnerships@abcam.com