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| ReagentType: | PCRMix |
| 100%Guarantee: | 6months |
Description
MethylampHotTaqPowerMixisapremixedready-to-loadsolutioncontaining:allreagentsrequiredforPCR(excepttemplate,primersandwater),compoundneededfordirectloADIngontoagarosegelandtwotrackingdyestofollowtheelectrophoresis.MethylampHotTaqPowerMixcontainstwoenzymes–MethylampHotTaqDNApolymeraseandaproofreadingpolymerase.Theseenzymestogetherhaveboththe 5’→3’ xonucleaseactivityaswellasthe 3’→5’ proofreadingactivity.
Composition
- MethylampHotTaqDNAPolymerase
- Proofreadingenzyme
- 5xReactionBuffer
- 12.5mMMgCl2:1xPCRsolution–2.5mMMgCl2
- 2mMdNTPsofeach:1xPCRsolution–200µMdATP,200µMdCTP,200µMdGTPand200µMdTTP
- BSA
- Bluedye:Migrationequivalentto3.5-4.5kbDNAfragment
- Yellowdye:Migrationrateinexcessofprimersin1%agarosegel:<35-45bp
- Compoundthatincreasessampledensityfordirectloading
- 1mlofmixissufficientfor250reactions
Applications
- HotStart
- PCRProofreading
StorageConditions
Routinestorage:-20°C.Shippingandtemporarystorageforupto1monthatroomtemperatureorstorageforupto6monthsat2-8°ChasnodetrimentaleffectsonthequalityofMethylampHotTaqPowerMix.


