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实时荧光定量PCR技术原理是什么?
mayitao
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SciencellsGeneQuery™HumanTie2/AngptSignalingqPCRArrayKit(GQH-TEK)isdesignedtofacilitategeneexpressionprofilingof88keygenesinvolvedinTie2signalingtransduction,whichisakeycomponentintheformationofbloodvesselsduringangiogenesis. Briefexamplesofhowincludedgenesmaybegroupedaccordingtofunctionareshownbelow:
- Ligands&receptors: ANGPT1,ANGPT2,TEK,TIE1
- Morphology: GRB2,PTPN11,SOS1,PTK2,RHOA
- Motility: PLD1,MAP2K1,DOK2,NCK1,CRK
- Transcriptionfactors: STAT1,STAT5B,MEF2A,FOXO3,EGR1
- Cellcycleregulation: CDC42,NFKB1,TNIP2,RIPK1,DIABLO
GeneQuery™qPCRarraykitsareqPCRreadyina96-wellplateformat,witheachwellcontainingoneprimersetthatrecognizesandefficientlyamplifiesaspecifictargetgenesCDNA.Thecarefullydesignedprimersensurethat:(i)theoptimalannealingtemperatureinqPCRanalysisis65°C(with2mMMg2+andnoDMSO);(ii)theprimersetrecognizesallknowntranscriptvariantsofthetargetgene,unlessotherwisenoted;and(iii)onlyonegeneisamplified.EachprimersethasbeenvalidatedbyqPCRwithmeltcurveanalysisandgelelectrophoresis.
GeneQuery™HumanTie2/AngptSignalingqPCRArrayPlateLayout*(8controls inBoldandItalic)
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | ADSS | CDC42 | DOK2 | FOXO3 | HIF1A | ITGB5 | MMP2 | PDIA3 | PTPN11 | SELE | TEK | β-actin |
B | AKT1 | CDKN1A | DOK4 | FYN | HRAS | KLF2 | MTOR | PGK1 | PXN | SELP | TIAM1 | GAPDH |
C | AKT2 | CRK | EGR1 | GJA5 | ICAM1 | MAP2K1 | NCK1 | PIK3CA | RAC1 | SOS1 | TIE1 | LDHA |
D | ALDOA | CTNNB1 | ELF2 | GRB14 | ILK | MAPK1 | NFKB1 | PIK3R1 | RAF1 | SPHK1 | TLN1 | NONO |
E | ANGPT1 | CXCL12 | ELK1 | GRB2 | ITGA5 | MAPK14 | NOS3 | PIK3R2 | RASA1 | STAT1 | TNIP2 | PPIH |
F | ANGPT2 | DIABLO | EPAS1 | GRB7 | ITGAV | MAPK3 | NOX1 | PLCG1 | RELA | STAT3 | TXNRD1 | GDC |
G | ARHGAP5 | DIAPH1 | FES | GYS1 | ITGB1 | MAPK7 | NR4A1 | PLD1 | RHOA | STAT5A | VCAM1 | PPC |
H | BIRC5 | DLL4 | FOXO1 | HAGH | ITGB3 | MEF2A | PAK1 | PTK2 | RIPK1 | STAT5B | VEGFA | NTC |
*geneselectionmaybeupdatedbasedonnewresearchanddevelopment
| CatalogNo. | GK029 |
|---|---|
| CountryofManufacture | UnitedStates |
| ProductCode | GQH-TEK |
| Size/Quantity | One96-wellplate,one20ulreactionperwell |
| ProductUse | GQH-TEKisforresearchuseonly.Itisnotapprovedforhumanoranimaluseorforapplicationinclinicalorinvitrodiagnosticprocedures. |
| Storage | Storeat4°Cuponreceipt.Forlong-termstorage(>1year),storetheplateat-20°Cinamanualdefrostfreezer. |
| ShippingInfo | Ambienttemperature. |
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