细胞凋亡常见7种检测方法大汇合相关交流-蚂蚁淘在线

细胞凋亡常见7种检测方法大汇合

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alpha-TubulinAntibodyProperties

Description

Anti-alpha-Tubulin(RABBIT)Antibody-600-401-880S

Host

Rabbit

TargetSpecies

Human

KnownCrossReactivity

human,mouse,rat.chicken,bovine

Clonality

Polyclonal

Application

ELISA:1:5,000
IFMicroscopy:1:500-1:2,000
WesternBlot:1:500-1:3,000
Immunohistochemistry:1:500-1:2,000
OtherDilution:UserOptimized

PhysicalState

Liquid(sterilefiltered)

ShippingCondition

DryIce

Format

IgG

Label

Unconjugated

Concentration

1.1mg/mLbyUVabsorbanceat280nm

Buffer

0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2

Preservative

0.01%(w/v)SodiumAzide

Stabilizer

None

GeneName

TUBA1B

RelevantLinks

NCBI-17986283
UniProtKB-P68363

alpha-TubulinAntibodyDescription

Background

TubulinLoadingControlAntibodyrecognizesmicrotubuleswhichareinvolvedinawidevarietyofcellularactivitiesrangingfrommitosisandtransporteventstocellmovementandthemaintenanceofcellshape.Tubulinitselfisaglobularproteinconsistingoftwopolypeptides(alphaandbetatubulin).Alphaandbetatubulindimersareassembledto13protofilamentsthatformamicrotubuleof22-nmdiameter.TyrosineligaseaddsaC-terminaltyrosinetomonomericalphatubulin.Assembledmicrotubulescanagainbedetyrosinatedbyacytoskeleton-associatedcarboxypeptidase.DetyrosinatedalphatubulinisreferredtoasGlu-tubulin.Anotherpost-translationalmodificationofdetyrosinatedalphatubulinisC-terminalpolyglutamylation,whichischaracteristicofmicrotubulesinneuronalcellsandthemitoticspindle.Thisantibodymakesanexcellentloadingcontrol.

Synonyms

rabbitanti-alpha-TubulinAntibody,rabbitanti-a-tubulinantibody,Tubulinalpha-1Bchain,Tubulinalpha-ubiquitouschain,Alpha-tubulinubiquitous,TubulinK-alpha-1,TUBA1B,tubulinloadingcontrol,Alpha-tubulin,Tubulinalpha-1A,TUBA1A,TUBA3,LIS3

Immunogen

Anti-TubulinLoadingControlAntibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoaminoacids427-441ofHumanalphaTubulin.

ImmunogenType

Peptide

StorageCondition

Storevialat-20°Corbelowpriortoopening.Thisvialcontainsarelativelylowvolumeofreagent(25µL).Tominimizelossofvolumedilute1:10byadding225µLofthebufferstatedabovedirectlytothevial.Recap,mixthoroughlyandbrieflycentrifugetocollectthevolumeatthebottomofthevial.Usethisintermediatedilutionwhencalculatingfinaldilutionsasrecommendedbelow.Storethevialat-20°Corbelowafterdilution.Avoidcyclesoffreezingandthawing.

ApplicationNote

Anti-TubulinAntibodyhasbeentestedforuseinELISAandwesternblot.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectabandat~50kDainsizecorrespondingtoalphatubulinbywesternblottinginmostcelllysatesorextracts.

Purity/Specifity

Anti-TubulinLoadingControlAntibodyisdirectedagainsthumanalphaTubulinprotein.TheLoadingControlAntibodywasaffinitypurifiedfrommonospecificantiserumbyimmunoaffinitypurification.ABLASTanalysiswasusedtosuggestthatthisantibodywouldreactwithalphaTubulinfromawiderangeoforganisms,includingavian,mammalianaquatic,parasiticandalgasourcesbasedon100%homologyfortheimmunogensequence.Crossreactivitywilloccurwithallisoformsofalphatubulin.Suchbroadreactivitymakesthisantibodyusefulasanexcellentloadingcontrol.

DisclaimerNote-General

Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.Theprospectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.

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