【正牌签约代理】creative biomart

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 MouseAnti-GAPDH(3E12)-LoadingControl antibody

 

产品编号	hz-0978M
英文名称	GAPDH(3E12)-LoadingControl
中文名称	3-***酸甘油醛脱氢酶单克隆抗体(内参抗体)
别    名	38kDaBFA-dependentADP-ribosylationsubstrate;Aging-associatedgene9protein;BARS-38;cb609;EC1.2.1.12;G3PD;G3PDH;GAPD;Glyceraldehyde3phosphatedehydrogenase;Glyceraldehyde3phosphatedehydrogenaseliver;Glyceraldehyde3phosphatedehydrogenasemuscle;KNC-NDS6;MGC102544;MGC102546;MGC103190;MGC103191;MGC105239;MGC127711;MGC88685;OCAS,p38component;OCT1coactivatorinSphase,38-KDcomponent;wu:fb33a10.
产品类型	内参抗体
研究领域	肿瘤  免疫学  神经生物学  信号转导  通道蛋白  新陈代谢  Alzheimers
抗体来源	Mouse
克隆类型	Monoclonal
克 隆 号	3E12
交叉反应	Human, Mouse, Rat, Rabbit,
产品应用	WB=1:500-5000 ELISA=1:1000-5000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） notyettestedinotherapplications.optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
分 子 量	38kDa
细胞定位	细胞核 细胞浆 细胞膜
性    状	LyophilizedorLiquid
浓    度	1mg/ml
免 疫 原	FulllengthGAPDHproteinpurifiedfromrabbitmuscle:
亚    型	IgG
纯化方法	affinitypurifiedbyProteinG
储 存 液	0.01MTBS(pH7.4)with1%BSA,0.03%Proclin300and50%Glycerol.
保存条件	Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.
PubMed	PubMed
产品介绍	background:LoadingControl
产品图片	Sample: Placenta(Mouse)Lysateat40ug293TCellLysateat40ugPrimary:Anti-GAPDH(bsm-0978M)at1/1000dilutionSecondary:IRDye800CWGoatAnti-RabbitIgGat1/20000dilutionPredictedbandsize:38kDObservedbandsize:38kD Sample:Bladder(Rat)Lysateat40ugPrimary:Anti-GAPDH(bsm-0978M)at1/300dilutionSecondary:IRDye800CWGoatAnti-RabbitIgGat1/20000dilutionPredictedbandsize:38kDObservedbandsize:38kD Sample:Liver(Mouse)Lysateat40ugPrimary:Anti-GAPDH(bsm-0978M)at1/300dilutionSecondary:IRDye800CWGoatAnti-MouseIgGat1/20000dilutionPredictedbandsize:38kDObservedbandsize:38kD Sample: Cerebrum(Mouse)Lysateat40ugPrimary:Anti-GAPDH(3E12)-LoadingControl(bsm-0978M)at1/1000dilutionSecondary:IRDye800CWGoatAnti-MouseIgGat1/20000dilutionPredictedbandsize:38kDObservedbandsize:38kD Paraformaldehyde-fixed,paraffinembedded(Ratpancreas);Antigenretrievalbyboilinginsodiumcitratebuffer(pH6.0)for15min;Blockendogenousperoxidaseby3%hydrogenperoxidefor20minutes;Blockingbuffer(normalgoatserum)at37°Cfor30min;Antibodyincubationwith(GAPDH(3E12)-LoadingControl)PolyclonalAntibody,Unconjugated(bsm-0978M)at1:500overnightat4°C,followedbyaconjugatedsecondary(sp-0023)for20minutesandDABstainin

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