1. Monolayer format for homogenous differentiation
2. Beating sheet or clusters as early as 7 days
3. Reproducible in multiple cell lines
4. Protocol as easy as A – B – C

PSdif-Cardio® is designedfor cardiomyocyte differentiation of human pluoripotent stem cells (ESC or iPSC) grown as a monolayer culture. The kit contains all necessary serum-free media formulated with polypeptidedifferentiation factors and modulators of the key cardiogenic pathways for a3-step derivation of beating cardiomyocytes.It is optimized to use with the serum-free and feeder-free growth medium PSGro® (StemRD cat# PGro). In independent beta testing of multiple human ESC and iPSC lines, the kit consistently generated cardiomyocytes at efficiencies higher than published monolayer protocols.