Stemrd/PDGF-bb,人/1000µg/PDGF-1000

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¥59980.00
货号:PDGF-1000
浏览量:127
品牌:Stemrd
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商品描述
SourceE. coliM.W.25 kDaCAS No.
Structural Info
FormulationLyophilized from sterile filtered PBS solution, carrier-free.
ReconstitutionBefore reconstitution, we recommend a brief spin to drivedown any material dislodged from the bottom of the tube. It is recommended to reconstitute the lyophilized product inacid solution such as 1mM HCl. Furtherdilutions should be made in sterile buffer or medium containing carrierproteins, such as albumin or serum.
StabilityThe lyophilized protein is stable for at least 1 yearif stored at -80 °C. Reconstituted protein is stable for at least 2 week at 4 ° C, but shouldbe stored in aliquots at -80 °C for longer term. Avoidrepeated freeze/thaw.
PurityGreater than 95% by SDS-PAGE analysis
Biological ActivityThe activity was determined by proliferation of the NIH3T3 cells.The EC50 ranges from 1 – 5 ng/ml.
Country of OriginUSA

Platelet-Derived Growth Factor (PDGF) is a mitogen for manycell types, such as fibroblast, smooth muscle cells and endothelial cells. StemRD produces PDGF-bb in Escherichia coli as a disulfide bond-linked homodimer with amolecular mass of 25 kDa. Thisproduct is suitable for tissue culture uses.

Product Sheet
Stemrd的WNT-5a属于激活“非规范”途径的WNT蛋白类别。小鼠WNT-5a的预测大小是一种包含356个氨基酸残基的单体蛋白。由于糖基化作用,在非还原条件下,通过SDS-PAGE分析,它以〜45 kDa的表观分子量迁移。StemRD的产品是从小鼠细胞系表达而来,并通过不同于公开方法的专有工艺进行纯化。