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品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
| Reactivity | Human |
|---|---|
| Clonality | Monoclonal , HIS.H8 |
| Host | Mouse |
| Isotype | IgG2b |
| Form | Liquid, 1mg/ml in 10mM PBS, pH 7.2 with 0.05% NaN3 |
| Application | WB - 1:1000 ~ 1:3000 |
| IP - 1:50 ~ 1:200 | |
| IF/ICC - 1:200 ~ 1:500 |
Epitope tags provide a method to localize gene products
in a variety of cell types, study the topology of proteins
and protein complexes, identify associated proteins, and
characterize newly identified, low abundance or poorly
immunogenic proteins when protein specific antibodies
are not available. Expression of recombinant proteins
in E-coli as a fusion protein with neighboring histidine
residues is one of the most popular methods of epitope
tags.
The affinity of the histidine-tag motif to Ni2+ by chelation
is strong and selective enough to enable purification of
the protein tohomogeneity by affinity chromatography
on a Ni2+ -NTA adsorbent.
