Description:	Live cell studies of cellular DNA content and cell cycle distribution are useful to detect variations of growth patterns due to a variety of physical, chemical, or biological means, to monitor apoptosis, and to study tumor behavior and suppressor gene mechanisms. In a given population, cells are distributed among three major phases of cell cycle: G0/G1 phase (one st of paired chromosomes per cell), S phase (DNA synthesis with variable amount of DNA), and G2/M phase (two sets of paired chromosomes per cell, prior to cell division). DNA content can be measured using fluorescent, DNA-selective stains that exhibit emission signals proportional to DNA mass. Flow cytometric analysis of these stained populations is then used to produce a frequency histogram that reveals the various cell cycle phases. This analysis is typically performed on permeabilized or fixed cells using a cell-impermeant nucleic acid stain, but is also possible using live cells and a cell-permeant nucleic acid stain. The CellCycle™ Blue stain is DNA-selective, cell membrane-permeant, and nonfluorescent stain for DNA content analysis in living cells. The CellCycle™ Blue stain is fluorescent upon binding to double-stranded DNA. CellCycle™ Blue stain is excited using both UV and 405 nm laser with emission ~460 nm. The staining protocol is simple and includes incubating suspended cells in the presence of CellCycle™ stain and directly measuring the fluorescence without the need for any additional treatment or centrifugation steps. This live cell stain allows the simultaneous co-staining of the cell population for other parameters, and allows for the possibility of cell sorting based on DNA content.
Order #:	A053-ABP
Unit Size:	100 assays
Supplier:	ABP Biosciences
Shipping:	RT
Storage:	2~8℃
Subcategory:	Cell Cycle
More information:	Go to webpage

   苏州蚂蚁淘生物技术有限责任公司是一家2001成立的经营生命科学用品的高科技企业，除了代理国际*公司生产的试剂、仪器、设备和耗材等产品外，也把寻找新的肿瘤标志物定位为公司的长远发展方向。 代理和经销的产品分为两类：医学类产品和生物类产品。医学类产品主要包括：肿瘤早期诊断试剂（意大利Xeptagen），甲状腺类检测产品（德国Medipan），过敏原检测系统（美国Hitachi），载玻片离心机及染色仪（德国Tharmac），高强聚焦超声刀（中国 希复康）。生物类产品主要包括：免疫学和神经生物学产品（以色列Alomone），细胞因子（以色列Prospec），生物化学和分子生物学类产品（美国Qbiogene），酶免分析试剂盒（美国Cytimmune），核酸纯化类产品（美国Omega），生物化学和免疫类产品（美国Alexis），酶免分析试剂盒和各种抗体（美国Cytimmune），多种抗原和抗体（美国Fitzgerald），细胞和分子生物学产品（美国Cambrex）等30多种。   order# description amount BMEG02 Bacillus megaterium protoplasts ready for transformation (strain WH320;1) Material is sufficient for 4 transformations plus control experiment. 5 x 500 µl BMEG50 Bacillus megaterium protoplasts, strain MS941 5 x 500 µl BMEG31 pC-His1623hp shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 μg BMEG32 pN-His-TEV1623hp shuttle vector, N-term. His-tag,TEV-site 10 μg BMEG30 p3STOP1623hp, shuttle vector, lyophilized DNA 10 μg BMEG33 pSPLipA-hp, shuttle vector with signal sequence, lypophilized DNA 10 μg BMEG34 pSPYocH-hp, shuttle vector with signal sequence, lypophilized DNA 10 µg BMEG03 pWH1520 shuttle vector, original; lyophilized DNA 5 µg BMEG10 pMM1522 shuttle vector, improved; lyophilized DNA 10 µg BMEG11 pMM1525 shuttle vector with signal sequence; lyophilized DNA 10 µg BMEG12 pHIS1522 shuttle vector, 6xHis-tagged; lyophilized DNA precursor of BMEG20 10 µg BMEG13 pHIS1525 shuttle vector with signal sequence; 6xHis-tagged; lyophilized DNA 10 µg BMEG14 pSTREP1525 shuttle vector with signal sequence; Strep-tagged; lyophilized DNA 10 µg BMEG15 pSTREPHIS1525 shuttle vector with signal sequence; Strep/6xHis double-tagged; lyophilized DNA 10 µg BMEG20 pC-His1622 shuttle vector, C-term. 6xHis-tag; lyophilized DNA 10 µg BMEG21 pC-Strep1622 shuttle vector, C-term. Strep-tag; lyophilized DNA 10 µg BMEG22 pN-His-TEV1622 shuttle vector, N-term. His-tag,TEV-site 10 µg BMEG23 pN-Strep-TEV1622 shuttle vector, N-term. Strep-tag,TEV-site 10 µg BMEG24 pN-StrepXa1622 shuttle vector, N-term. Strep-tag; Xa site; lyophilized DNA 10 µg BMEG25 pSTOP1622, shuttle vector, lyophilized DNA 10 µg BMEGT702 Bacillus megaterium protoplasts, strain MS941, pretransformed with pT7-RNAP 5 x 500µL BMEGT701 Bacillus megaterium high yield T7 gene expression kit,  includes pretransformed protoplasts BMEGT702 (5 x 500µl), pPT7 cloning vector and pPT7-GFP control vector (vectors lyophilized, 10 µg each) Kit BMEGT710 Bacillus megaterium pPT7 cloning vector, lyophilized 10 µg shipped at RT, protoplasts shipped on dry ice  store lyophilized vectors at 4°C, reconstituted vectors at -20°C, protoplasts at -70°C All vectors are E. coli / B. megaterium shuttle vectors Please note that protoplasts of Bacillus megaterium have a limited shelf life of some months only, depending on the date of production. Therefore purchase and use of protoplasts must be thoroughly planned.