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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
我公司长期供应常规性的生物试剂,耗材批发零售。秉承质量第一 信誉至上 售后完善 以客户标准为原则,为新老客户提供一个值得信任的平台。各种优势产品:life (脂质体2000,3000),Gelred(核酸染料) 。Trizol,TRIzol® LS 。罗氏潮霉素B。 二甲基亚砜(DMSO)。HyClone培养基。gibco 双抗 胰酶 B27 胎牛血清( 16000-044) 胎牛血清( 10099-141) 等。 CST ,ABcam ,santa抗体。日本同仁CCK-8试剂盒。西班牙琼脂糖。另有 sigma,amresco原装试剂大量现货。以上报价均为市场报价,仅供参考!欢迎新老客户咨询选购!18915418616

- 药准字:
- 主要组成成分:mobitec的巨大芽孢杆菌表达系统
- 性状:mobitec的巨大芽孢杆菌表达系统
- 适应症:mobitec的巨大芽孢杆菌表达系统
- 规格:mobitec的巨大芽孢杆菌表达系统
- 贮藏:mobitec的巨大芽孢杆菌表达系统
- 有效期:36
- 生产企业:德国
hp-vector表达系统megaterium芽孢杆菌
hp-vector expression systems for bacillusmegaterium
features:
high performance vectors with optimized sequence
protein yield up to 10 times enhanced compared toprotein expression with basic plasmid
plasmids with established mcs
encoding c- or n-terminal his-tag for versatilepurification (native, 6xhis-tag)
secretion with lipa or yoch signal peptide up to 9fold increased
optimized high performance (hp) vectors for bacillusmegaterium!
mobitec provides a wide range of useful vectors forthe b. megaterium system that are adaptable to most applicationsand protein purification methods. these include vectors carrying asecretion signal peptide sequence and a 6xhis-tag.
with our new hp-vectors yields up to 10-fold highercompared to the basic plasmids are possible. all plasmids haveestablished multiple cloning sites (mcs) for versatile cloning.furthermore we offer vectors encoding c- or n-terminal his-tags foreasy purification. the protein secretion with lipa or yoch signalpeptides is up to nine-fold increased. induction of proteinexpression of all vectors is achieved by the tightly regulated andefficiently inducible xylose operon.
versatile system with a wide range of vectors
the b. megaterium expression system provides aversatile and easy-to-handle tool for stable and high-yield proteinproduction, both small- and large-scale.
b. megaterium has proven to be an excellentalternative host to e. coli for heterologous gene expression.unlike other bacilli strains, proteolytic degradation by alkalineproteases is avoided. in addition, there are no endotoxins found inthe cell wall.
b. megaterium protoplasts specifically optimized fortransformation
the protoplasts supplied by mobitec are from b.megaterium strain wh320, developed by prof. dr. w. hillen at theinstitute of microbiology in erlangen, germany. these protoplastsare prepared according to an optimized protocol resulting in thehighest transformation efficiencies.
for secretion vectors we offer protoplasts of strainms941. both strains are mutants of dsm319, where wh320 is achemically mutant, while ms941 has a defined deletion in the geneof the major extracellular protease nprm.
order information, shipping and storage
please click on the respective order # for furtherdetails.
order# description amount
bmeg02 bacillus megaterium protoplasts ready fortransformation (strain wh320;1)
material is sufficient for 4 transformations pluscontrol experiment. 5 x 500 µl
bmeg50 bacillus megaterium protoplasts, strain ms9415 x 500 µl
bmeg31 pc-his1623hp shuttle vector, c-term.6xhis-tag; lyophilized dna 10 μg
bmeg32 pn-his-tev1623hp shuttle vector, n-term.his-tag,tev-site 10 μg
bmeg30 p3stop1623hp, shuttle vector, lyophilized dna10 μg
bmeg33 psplipa-hp, shuttle vector with signalsequence, lypophilized dna 10 μg
bmeg34 pspyoch-hp, shuttle vector with signalsequence, lypophilized dna 10 µg
shipped at rt, protoplasts shipped on dry ice
store lyophilized vectors at 4°c, reconstitutedvectors at -20°c, protoplasts at -70°c
all vectors are e. coli / b. megaterium shuttlevectors
please note that protoplasts of bacillus megateriumhave a limited shelf life of some months only, depending on thedate of production. therefore purchase and use of protoplasts mustbe thoroughly planned.
蚂蚁淘 2018-02-18
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|---|---|---|---|
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