ThismaterialisofferedbyShanghaiPrimeGeneBio-Techforresearch,laboratoryorfurtherevaluationpurposes.NOTFORHUMANUSE.
VEGFwasinitiallypurifiedfrommediaconditionedbynormalbovinepituitaryfolliculo-stellatecellsandbyavarietyoftransformedcelllinesasamitogenspecificforvascularendothelialcells.ItwassubsequentlyfoundtobeidenticaltoanindependentlydiscoveredvascularpermeABIlityfactor(VPF),whichwaspreviouslyidentifiedinmediaconditionedbytumorcelllinesbasedonitsabilitytoincreasethepermeabilityofcapillarybloodvessels.ThreemouseCDNAclones,whicharisethroughalternativesplicingandwhichencodematuremousemonomericVEGFhaving120,164,or188,aminoacids,respectively,havebeenidentified.Tworeceptortyrosinekinases(RTKs),Flt-1andFlk-1(themousehomologueofhumanKDR),bothmembersofthetypeIIIsubclassofRTKscontainingsevenimmunoglobulin-likerepeatsintheirextracellulardomains,havebeenshowntobindVEGFwithhighaffinity.TherolesofthehomodimersofKDR,Flt,andtheheterodimerofKDR/FltinVEGFsignaltransductionremaintobeelucidated.Invivo,VEGFhasbeenfoundtobeapotentangiogenesisinducer.

Specifications:

CatalogNo.	125-07
CountryofManufacture	UnitedStates
ProductCode	rMuVEGF165
Size/Quantity	2μg
ProductUse	Thisproductisforresearchuseonly.Itisnotapprovedforuseinhumans,animals,orinvitrodiagnosticprocedures.
Storage	Thislyophilizedpreparationisstableat2-8°C,butshouldbekeptat-20°Cforlongtermstorage,preferablydesiccated.Uponreconstitution,thepreparationisstableforuptooneweekat
ShippingInfo	Dryice
AASequence	MAPTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Source	Escherichiacoli.
MolecularWeight	RecombinantmurineVEGF165isa39.0kDadisulfide-linkedhomodimericproteinconsistingoftwo165aminoacidpolypeptidechains.
Purity	>95%bySDS-PAGEandHPLCanalyses.
BIOLOGicalActivity	Measuredbyitsabilitytostimulate3H-thymidineincorporationinHUVEcells.TheED50forthiseffectistypically2-4ng/mL.
PhysicalAppearance	SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation	Lyophilizedfroma0.2?mfilteredsolutioninPBS,pH7.4.
Endotoxin	Lessthan1EU/μgofrmVEGF165asdeterminedbyLALmethod.
Reconstitution	Werecommendthatthisvialbebrieflycentrifugedpriortoopeningtobringthecontentstothebottom.Reconstituteinsteriledistilledwateroraqueousbuffercontaining0.1%BSAtoaconcentrat

SciencCell公司 是1999年成立于美国洛杉矶圣地亚哥，是一家快速成长的生物技术公司，其一直专注于是研发实验和治疗相关的细胞产品。ScienCell公司提供多种多样、高质量的人和动物的原代细胞，原代细胞培养基、干细胞研究相关领域产品和相关试剂。