EachprimersetincludedinGQH-CEqPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters

Sciencell"sGeneQuery™HumanCDNAEvaluationKit(GQH-CE)assessescDNAquality.ThekitverifiessuccessfulreversetranscriptionofmessengerRNA(mRNA)tocomplementaryDNA(cDNA)andrevealsthepresenceofgenomicDNA(gDNA)contaminationincDNAsamples.GoodqualitycDNAisacriticalcomponentforsuccessfulgeneexpressionprofiling.TheGQH-CEkitishighlyrecommendedforcDNAapplicationssuchasGeneQuery™qPCRarrays.

EachprimersetincludedinGQH-CEqPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg²⁺,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.EachprimersethasbeenvalidatedbyqPCRbymeltcurveanalysisandgelelectrophoresis. 

QualityControl

EachprimersetisvalidatedbyqPCRmeltcurveandamplificationcurveanalyses.ThePCRproductsareanalyzedbygelelectrophoresistoconfirmsinglebandamplification.

Specifications:

CatalogNo.	GK992
CountryofManufacture	UnitedStates
ProductCode	GQH-CE
Size/Quantity	One96-wellplate,one20ulreactionperwell
ProductUse	GQH-CEisforresearchuseonly.Itisnotapprovedforhumanoranimaluseorforapplicationinclinicalorinvitrodiagnosticprocedures.
Storage	Thisproductisshippedatambienttemperature.Uponreceipt,thevialsshouldbestoredat4°Candaregoodforupto12months.Forlong-termstorage(>1year),storethevialsat-20°Cinamanualdefrostfreezer.
ShippingInfo	Ambienttemperature.

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