EachprimersetincludedinGQH-CEDqPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters

Sciencell"sGeneQuery™HumanCDNAEvaluationKit,Deluxe(GQH-CED)assessescDNAquality.ThekitverifiessuccessfulreversetranscriptionofmessengerRNA(mRNA)tocomplementaryDNA(cDNA),revealsthepresenceofgenomicDNA(gDNA)contaminationincDNAsamples,anddetectsqPCRinhibitorcontamination.GoodqualitycDNAisacriticalcomponentforsuccessfulgeneexpressionprofiling.TheGQH-CEDkitishighlyrecommendedforcDNAapplicationssuchasGeneQuery™qPCRarrays.

EachprimersetincludedinGQH-CEDqPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.EachprimersethasbeenvalidatedbyqPCRbymeltcurveanalysisandgelelectrophoresis.

QualityControl

EachprimersetisvalidatedbyqPCRmeltcurveandamplificationcurveanalyses.ThePCRproductsareanalyzedbygelelectrophoresistoconfirmsinglebandamplification.

Specifications:

CatalogNo.	GK991
CountryofManufacture	UnitedStates
ProductCode	GQH-CED
Size/Quantity	100Reactions
ProductUse	GQH-CEDisforresearchuseonly.Itisnotapprovedforhumanoranimaluseorforapplicationinclinicalorinvitrodiagnosticprocedures.
Storage	Thisproductisshippedatambienttemperature.Uponreceipt,thevialsshouldbestoredat4°Candaregoodforupto12months.Forlong-termstorage(>1year),storethevialsat-20°Cinamanualdefrostfreezer.
ShippingInfo	Ambienttemperature.

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