EachprimersetincludedinHMSC-C-qPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon. 

Humanmesenchymalstemcells(MSCs)areapopulationofmultipotentcellsthatcanbedifferentiatedintomultiplelineage-specificcells,whichcanformbone,fat,cartilage,muscleandtendon.Theprocessofcartilageformationfromcondensedmesenchymetissueisknownaschondrogenesis.SciencellhascreatedaconvenientqPCRkitfortheassessmentofhumanmesenchymalstemcellchondrogenesis.SOX9andCOL2A1qPCRprimersincludedinthekitallowforthedetectionandquantificationofearly-to-mid-andlate-stageofhumanMSCchondrogenesis,respectively¹.

Note:allgenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).

FourqPCRcontrolsareincludedinthiskittoverifysuccessfulreversetranscriptionofmessengerRNA(mRNA)tocomplementaryDNA(CDNA),revealthepresenceofgenomicDNA(gDNA)contaminationincDNAsamples,anddetectqPCRinhibitorcontamination.GoodqualitycDNAisacriticalcomponentforsuccessfulgeneexpressionanalysis.

EachprimersetincludedinHMSC-C-qPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.EachprimersethasbeenvalidatedbyqPCRbymeltcurveanalysisandgelelectrophoresis.

Specifications:

CatalogNo.	8328
CountryofManufacture	UnitedStates
ProductCode	HMSC-C-qPCR
Size/Quantity	50reactions
ProductUse	HMSC-CPCRkitisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures.
Storage	Storeat-20°Cuponreceipt.Avoidrepeatedfreezethawcyclesbymakingsixaliquotsat110μLeach.
ShippingInfo	Dryice.
References	[1]Sekiya,I.,Vuoristo,J.T.,Larson,B.L.,&Prockop,D.J.(2002).Invitrocartilageformationbyhumanadultstemcellsfrombonemarrowstromadefinesthesequenceofcellularandmoleculareventsduringchondrogenesis.PNAS,99(7),4397-4402.

SciencCell公司 是1999年成立于美国洛杉矶圣地亚哥，是一家快速成长的生物技术公司，其一直专注于是研发实验和治疗相关的细胞产品。ScienCell公司提供多种多样、高质量的人和动物的原代细胞，原代细胞培养基、干细胞研究相关领域产品和相关试剂。