FourqPCRcontrolsareincludedinthiskittoverifysuccessfulreversetranscriptionofmessengerRNA(mRNA)tocomplementaryDNA(CDNA),revealthepresenceofgenomicDNA(gDNA)contaminationincDNAsamples,anddetectqPCRinhibitorcontamination.

Humanmesenchymalstemcells(MSCs)areapopulationofmultipotentcellsthatcanbedifferentiatedintomultiplelineage-specificcells,whichcanformbone,fat,cartilage,muscleandtendon.Amongthem,ADIpocytesareoneofthecelltypesthatcanbederivedfromMSCsthroughAdipogenesis.Theprocessoffatformationplaysaroleinobesity,cardiovasculardiseasesandmetabolicdisorders.SciencellhascreatedaconvenientqPCRkitfortheassessmentofhumanmesenchymalstemcelladipogenesis.CEBPB,PPARGandFABP4qPCRprimersincludedinthekitallowforthedetectionandquantificationofearly-,mid-andlate-stagehumanMSCadipogenesis,respectively.

Note:allgenenamesfollowtheirofficialsymbolsbytheHumanGenomeOrganizationGeneNomenclatureCommittee(HGNC).

FourqPCRcontrolsareincludedinthiskittoverifysuccessfulreversetranscriptionofmessengerRNA(mRNA)tocomplementaryDNA(cDNA),revealthepresenceofgenomicDNA(gDNA)contaminationincDNAsamples,anddetectqPCRinhibitorcontamination.GoodqualitycDNAisacriticalcomponentforsuccessfulgeneexpressionanalysis.

EachprimersetincludedinHMSC-A-qPCRkitarriveslyophilizedina2mLvial.Allprimersaredesignedandtestedunderthesameparameters:(i)anoptimalannealingtemperatureof65°C(with2mMMg2+,andnoDMSO);(ii)recognitionofallknowntargetgenetranscriptvariants;and(iii)specificamplificationofonlyoneamplicon.EachprimersethasbeenvalidatedbyqPCRbymeltcurveanalysisandgelelectrophoresis.

Specifications:

CatalogNo.	8298
CountryofManufacture	UnitedStates
ProductCode	HMSC-A-qPCR
Size/Quantity	50reactions
ProductUse	HMSC-APCRkitisforresearchuseonly.Notforuseinanimals,humans,ordiagnosticprocedures.
Storage	Storeat-20°Cuponreceipt.Avoidrepeatedfreezethawcyclesbymakingsixaliquotsat110μLeach.
ShippingInfo	Dryice.
References	[1]Menssen,A.etal.(2011).Differentialgeneexpressionprofilingofhumanbonemarrow-derivedmesenchymalstemcellsduringadipogenicdevelopment.BMCGenomics.12:461-477.

SciencCell公司 是1999年成立于美国洛杉矶圣地亚哥，是一家快速成长的生物技术公司，其一直专注于是研发实验和治疗相关的细胞产品。ScienCell公司提供多种多样、高质量的人和动物的原代细胞，原代细胞培养基、干细胞研究相关领域产品和相关试剂。