Isolatedfromembryonicday13CD1mouseembryos.Thesecellsarecryopreservedatprimarycultureanddeliveredfrozen.Eachvialcontains1x10^6cellsin1mlvolume.

MouseEmbryonicFibroblasts(MEF)areusedtosupportthegrowthofmouseandhumanpluripotentstemcells[1].MEFnotonlyprovideasubstrateforpluripotentstemcellstogrowon,butalsosecretecriticalgrowthfactorstomaintainstemcellpluripotency.MEFareisolatedfrommouseembryosandusedatearlypassages[2].Toserveasfeedercells,MEFmustbetreatedwithmitomycinCorbyirrADIationtopreventcellproliferation.Thetreatedcellscanalsobeusedtogenerateconditionedmediumforfeeder-freecultureofpluripotentstemcells.

MEFfromSciencellResearchLaboratoriesareisolatedfromembryonicday13CD1mouseembryos.Thesecellsarecryopreservedatprimarycultureanddeliveredfrozen.Eachvialcontains1x10^6cellsin1mlvolume.MEFarecharacterizedbyimmunofluorescencewithantibodyspecifictofibronectin.MEFarenegativeformycoplasma,bacteria,yeast,andfungi.MEFareguaranteedtofurtherexpandfor5populationdoublingsundertheconditionsprovidedbyScienCellResearchLaboratories.

RecommendedMedium

ItisrecommendedtouseDMEM(Cat.#09221)supplementedwith10%fetalbovineserum(FBS,Cat.#0010,0025,0500)forculturingMEFinvitro.

Specifications:

CatalogNo.	M7540
CountryofManufacture
ProductCode	MEF
Size/Quantity	1x10^6cells/vial
ProductUse	MEFareusedasafeederlayerinmouseandhumanpluripotentstemcellculture.Theyareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures..
Storage	Uponreceiving,directlyandimmediatelytransferthecellsfromdryicetoliquidnitrogenandkeepthecellsinliquidnitrogenuntiltheyareneededforexperiments.
ShippingInfo	Dryice.
References	[1]BRADLEY,A.(1987).Productionandanalysisofchimaeras.InTeratocarcinomasandEmbryonicStemCells:APracticalApproach,(ed.E.J.Robertson),pp.113-151.Oxford:IRLPress.[2]Nagyetal.(2006)PreparingMouseEmbryoFibroblastsColdSpringHarborProtocols.2006:pdb.prot4398.

SciencCell公司 是1999年成立于美国洛杉矶圣地亚哥，是一家快速成长的生物技术公司，其一直专注于是研发实验和治疗相关的细胞产品。ScienCell公司提供多种多样、高质量的人和动物的原代细胞，原代细胞培养基、干细胞研究相关领域产品和相关试剂。