Isolatedfromfetalhumanskin.HDF-f-mtarecryopreservedatP0anddeliveredfrozen.Eachvialcontains>5x10^5cellsin1mlvolume.

Fibroblastsaremesenchymalcellsderivedfromtheembryonicmesoderm.Theyhavebeenextensivelyusedforawiderangeofcellularandmolecularstudiesastheyareoneofeasiesttypesofcellstogrowinculture.TheirdurABIlityalsomakesthemamenabletoavarietyofmanipulationsrangingfromstudiesemployinggenetransfectiontomicroinjection.Ingeneral,fibroblastssecreteanon-rigidextracellularmatrixwhichisrichintypeIand/ortypeIIIcollagen[1].Thereisevidenceshowingthatfibroblastsinvariousorgansareintrinsicallydifferent[2].Dermalfibroblastsswitchfromaproliferative,migratoryphasetoacontractile,matrix-remodelingphaseduringwoundhealing.Inaddition,theysecretelargequantitiesofhyaluronaninresponsetoinflammatorystimuli[3].

HDF-f-mtfromSciencellResearchLaboratoriesareisolatedfromfetalhumanskin.HDF-f-mtarecryopreservedatP0anddeliveredfrozen.Eachvialcontains>5x10^5cellsin1mlvolume.HDF-f-mtarecharacterizedbytheirspindlemorphologyandimmunofluorescencewithantibodyspecifictofibronectin.HDF-f-mtarenegativeforHIV-1,HBV,HCV,mycoplasma,bacteria,yeast,andfungi.HDF-f-mtareguaranteedtofurthercultureundertheconditionsprovidedbyScienCellResearchLaboratories;however,HDF-f-mtarenotrecommendedforexpandingasmitomycinCtreatmentpreventsfurthercellproliferation.

RecommendedMedium

ItisrecommendedtouseFibroblastMedium(FM,Cat.#2301)forculturingHDF-f-mtinvitro.

Specifications:

CatalogNo.	2350
CountryofManufacture	UnitedStates
ProductCode	HDF-f-mt
Size/Quantity	1x10^5cells/vial
ProductUse	HDF-f-mtareforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures.
Storage	Directlyandimmediatelytransfercellsfromdryicetoliquidnitrogenuponreceivingandkeepthecellsinliquidnitrogenuntilcellcultureneededforexperiments.
ShippingInfo	Dryice.
References	[1]Conrad,G.W.,Hart,G.W.,Chen,Y.(1977)Differencesinvitrobetweenfibroblast-likecellsfromcornea,heart,andskinofembryonicchicks.J.CellSci.26:119-137.[2]Gabbiani,G.,Rungger-Brandle,E.,Thefibroblast.InTissueRepairandRegeneration(L.E.Glynn,ed.),pp1-50.HandbookofInflammation,Vol.3.Amsterdam,Elsevier,1981.[3]StairS,CarlsonKW,ShusterS,WeiET,SternR(2002)Mystixinpeptidesreducehyaluronandepositionandedemaformation.EurJPharmacol30;450(3):291-6.

SciencCell公司 是1999年成立于美国洛杉矶圣地亚哥，是一家快速成长的生物技术公司，其一直专注于是研发实验和治疗相关的细胞产品。ScienCell公司提供多种多样、高质量的人和动物的原代细胞，原代细胞培养基、干细胞研究相关领域产品和相关试剂。