MHMafromSciencellResearchLaboratoriesareisolatedfrompostnatalday2mouseliver.MHMaarecryopreservedafterpurificationanddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.

Macrophagesarecellsdifferentiatedfromcirculatingbonemarrow-derivedmonocytes.ThemainfunctionofmacrophagesistoremovecellulardebrisanddestroyinvADIngpathogens.MouseHepaticMacrophages(MHMa),whicharealsoknownasKupffercells,residewithinthelumenofliversinusoids.MHMaprotecttheliverbyrespondingtopathogensandmetastaticcells,whiletoleratingharmlessselfandforeignantigens,whichenterviabloodflowthroughtheportalveinandhepaticartery[1].Recentstudieshaveshownthathepaticmacrophagesplayanimportantroleinfibrosis,liverinflammation,fattyliverdisease,andlivertransplantation[2-4].MHMaareanexcellentmodelforstudyingmacrophagefunctionsundernormalphysiologicalandpathologicalconditions.

MHMafromScienCellResearchLaboratoriesareisolatedfrompostnatalday2mouseliver.MHMaarecryopreservedafterpurificationanddeliveredfrozen.Eachvialcontains>1x10^6cellsin1mlvolume.MHMaarecharacterizedbyimmunofluorescencewithantibodytoF4/80.MHMaarenegativeformycoplasma,bacteria,yeast,andfungi.MHMaareguaranteedtofurthercultureintheconditionsprovidedbyScienCellResearchLaboratories;however,RHMaarenotrecommendedforexpandingorlong-termculturessincethecellsdonotproliferateinregularculture.

ItisrecommendedtouseMacrophageMedium(MaM,Cat.#1921)forculturingMHMainvitro.

Specifications:

CatalogNo.	M5340
CountryofManufacture	UnitedStates
ProductCode	MHMa
Size/Quantity	1x10^6cells/vial
ProductUse	MHMaareforresearchuseonly.Theyarenotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures.
Storage	Uponreceiving,directlyandimmediatelytransferthecellsfromdryicetoliquidnitrogenandkeepthecellsinliquidnitrogenuntiltheyareneededforexperiments.
ShippingInfo	Dryice.
References	[1]LiaskouE,WilsonDV,OoYH.(2012)"Innateimmunecellsinliverinflammation."MediatorsInflamm.2012:949157.[2]BieghsV,VerheyenF,vanGorpPJ,HendrikxT,WoutersK,LutjohannD,GijbelsMJ,FebbraioM,BinderCJ,HofkerMH,Shiri-SverdlovR.(2012)"InternalizationofmodifiedlipidsbyCD36andSR-AleadstohepaticinflammationandlysosomalcholesterolstorageinKupffercells."PLoSOne.7:e34378.[3]TianY,JochumW,GeorgievP,MoritzW,GrafR,ClavienPA.(2006)"Kupffercell-dependentTNF-alphasignalingmediatesinjuryinthearterializedsmall-for-sizelivertransplantationinthemouse."ProcNatlAcadSciUSA.103:4598-603.[4]SekiE,deMinicisS,InokuchiS,TauraK,MiyaiK,vanRooijenN,SchwabeRF,BrennerDA.(2009)"CCR2promoteshepaticfibrosisinmice."Hepatology.50:185-97

SciencCell公司 是1999年成立于美国洛杉矶圣地亚哥，是一家快速成长的生物技术公司，其一直专注于是研发实验和治疗相关的细胞产品。ScienCell公司提供多种多样、高质量的人和动物的原代细胞，原代细胞培养基、干细胞研究相关领域产品和相关试剂。