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question 【求助】NEB HIFI酶 双酶切
请问最近新买了NEB 的HiFi酶 分别是sac1 和Bamh1两种酶,说明书上写着是5分钟快速酶切。请问大家有没有尝试这种酶的,双酶切实际 时间和用量分别是多少呢?谢谢大家。
小刁315 2014-11-15
  • Try to use CutSmart buffer for Sac I (1 ul) to cut 1-5 ug of plasmid DNA at 37 C for 2 hours in a reaction with final volume around 20-50 ul.Set up a quite same reaction for BamH I to cut your plasmid DNA.
answer全部回答 (共 2 条)
  • Try to use CutSmart buffer for Sac I (1 ul) to cut 1-5 ug of plasmid DNA at 37 C for 2 hours in a reaction with final volume around 20-50 ul.Set up a quite same reaction for BamH I to cut your plasmid DNA.Thank you!