Description:

Product Source

An E. coli strain that carries the NciI gene from Neisseria cinerea (NRCC 31006).

Reagents Supplied

The following reagents are supplied with this product:

	Store at (°C)	Concentration
CutSmart® Buffer	-20	10X

Notes:

NciI produces DNA fragments that have a single-base 5´ extension which are more difficult to ligate than blunt-ended fragments.Cleavage of mammalian genomic DNA is impaired by overlapping CpG methylation.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch® PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.

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