Description	TLR2 / TLR4 Inhibitor Peptide: 1 mg (lyophilized); sequence: DRQIKIWFQNRRMKWKKPGFLRDPWCKYQML (Inhibitor sequence: PGFLRDPWCKYQML); Molecular weight: 4097.92 DaAntennapedia Control Peptide: 1 mg (lyophilized); sequence: DRQIKIWFQNRRMKWKK; Molecular weight: 2361 Da
Specificity	TLR2 and TLR4 Inhibitor Peptide: COBRA TLR2 and TLR4 Inhibitor interferes with interaction between TIRAP/Mal and TIR domain of TLR2 or TLR4.
PreparationMethod	Preparation of 2.5 mM peptide stock solutionsTLR2 / TLR4 Inhibitor Peptide: A final volume of 100 ul will make a 2.5 mM stock solution. Add 100 ul sterile water to the tube of peptide. Carefully pipet to ensure all of the peptide is dissolved and briefly spin the tube before opening.Antennapedia Control Peptide: A final volume of 170 ul will make a 2.5 mM stock solution. Add 170 ul sterile water to the tube of peptide. Carefully pipet to ensure all of the peptide is dissolved and briefly spin the tube before opening.The stock solutions may be diluted further to make working solutions. Dilute according to the needs for your assay. For example, dilute 2.5 mM stock solutions 1:10 in sterile 1X PBS or cell culture media to make 250 uM working solutions. Working solution should be made fresh daily and not be stored.
Gene	TIRAP

Application Notes

The inhibitor is used in assays to inhibit TLR2 and TLR4 signaling. We recommend an initial titration of the inhibitor from 0-50 uM for in vitro assays along with control of which concentrations should be mirror inhibitor concentrations. Inhibitor and control should be preincubated with cells prior to ligand activation to allow sufficient time for the peptides to enter from the media into the cell. We typically preincubate with inhibitor and control for 1 h prior to TLR2 or TLR4 activation (Figures 1 and 2); however, optimal preincubation times may vary between model systems. The TLR2/NF-kB/SEAPorter™ cell line and TLR4/MD2/CD14/NF-kB/SEAPorter™ cell line are a useful positive control model system for studying inhibition of TLR2 and TLR4 activation, respectively (Figures 1 through 3). Use in In vitro assay reported in scientific literature (PMID 26908090). Use in FLOW cytometry reported in scientific literature (PMID 27838489).

Publications

ReadPublications using NBP2-26245 in the following applications: FLOW 1 publication In vitro 1 publication

Novus Biologicals 美国Novus Biologicals公司是一家抗体专业公司，生产用于研究的单克隆和多克隆抗体，包含5万多种一抗和近1000种二抗，产品涉及神经生物学，细胞凋亡，肿瘤，DNA修复，干细胞标记，信号转导等多个研究方向。多年的抗体生产服务经验，使novus得以引领生物科学发展趋势，并可随时为生物科研工作者提供最新和最广泛的产品。Novus的愿景是通过开发和销售在前沿科学的独特产品来推动科学发现。Novus组织了商业化机制，致力于为生物研究界提供独特的生物材料。Novus还持续关注科学趋势，并为新的研究提供必要的材料。Novus的产品广泛使用于机构和商业研究，并在推动生物学研究中起着重要的。 Novus Biologicals is a biotech company based in Littleton, Colorado, USA with offices in San Diego, California, Oakville, ON, Canada and the Cambridge Science Park in Cambridge, UK.[1] The company develops, licenses and markets research materials to scientists in academic, corporate and government laboratories. Novus, like many others companies such as Abcam, Covance, Proteintech Group, EnCor Biotechnology and Millipore Corporation, arose to answer a call for research grade antibodies for the scientific market.Founded by Karen Padgett in 1996, Novus Biologicals has sought to partner with leading scientific researchers and third-party providers to develop an extensive range of more than 180,000 products.[2] The main products are antibodies, proteins, peptides, RNAi, cell lysates, isotype controls, slides, and kits. Padgett is company president and CEO, and has been active both in the academic and local Colorado communities. In 2008, Novus became a limited liability company when Mainsail Partners invested in the company.[4] Novus Biologicals has reported double digit revenue growth every year since its inception, has been selected six years in a row to the Inc. (magazine) 5000 fastest-growing private companies in America,[5] and was on the 2009 list of Colorado Companies to Watch.[6]In 2014 major antibody and reagent supplier Bio-Techne, acquired Novus Biologicals for $60 million.[7] The acquisition was meant to provide scientists and doctors a broader selection of products in one location. 产品列表： Novus-Biologicals SP4385P MC1 Receptor Antibody 0.05mg Novus-Biologicals PA184X Defensin beta 3 Protein 0.02mg Novus-Biologicals NSB998 PKA 2 beta [phospho Ser114] Antibody 0.1ml Novus-Biologicals NSB992 cAMP Protein Kinase Catalytic subunit beta [phospho Ser338] Antibody 0.05ml Novus-Biologicals NSB988 cAMP Protein Kinase Catalytic subunit alpha [phospho Thr197] Antibody 0.1ml Novus-Biologicals NSB979 PKC theta [phospho Ser695] Antibody 0.1ml Novus-Biologicals NSB978 PKC eta [phospho Ser674] Antibody 0.1ml Novus-Biologicals NSB977 PKC epsilon [phospho Ser729] Antibody 0.1ml Novus-Biologicals NSB976 PKC delta [phospho Ser664] Antibody 0.1ml Novus-Biologicals NSB975 PKC gamma [phospho Thr674] Antibody 0.1ml Novus-Biologicals NSB970 PKC theta [phospho Ser676] Antibody 0.1ml Novus-Biologicals NSB969 PKC eta [phospho Thr655] Antibody 0.1ml Novus-Biologicals NSB968 PKC iota [phospho Thr555/Thr563] Antibody 0.1ml Novus-Biologicals NSB966 PKC delta [phospho Ser645] Antibody 0.1ml Novus-Biologicals NSB965 PKC gamma [phospho Thr655] Antibody 0.1ml Novus-Biologicals NSB964 PKC beta [phospho Thr641] Antibody 0.1ml Novus-Biologicals NSB963 PKC beta [phospho Thr642] Antibody 0.1ml Novus-Biologicals NSB962 PKC alpha [phospho Thr638] Antibody 0.1ml Novus-Biologicals NSB961 PKC mu [phospho Ser738/Ser742] Antibody 0.1ml Novus-Biologicals NSB960 PKC theta [phospho Thr538] Antibody 0.1ml Novus-Biologicals NSB956 PKC gamma [phospho Thr514] Antibody 0.1ml Novus-Biologicals NSB955 PKC beta [phospho Thr500] Antibody 0.1ml Novus-Biologicals NSB948 GATA4 [phospho Ser105] Antibody 0.1ml Novus-Biologicals NSB944 NFAT1 [phospho Ser54] Antibody 0.1ml Novus-Biologicals NSB942 PAK1/2/3 [phospho Thr423] Antibody 0.1ml Novus-Biologicals NSB940 PAK1/2/3 [phospho Ser141] Antibody 0.1ml Novus-Biologicals NSB928 RSK1 [phospho Ser380] Antibody 0.1ml Novus-Biologicals NSB926 RSK1 [phospho Ser363] Antibody 0.1ml Novus-Biologicals NSB924 RSK1 [phospho Ser221] Antibody 0.1ml Novus-Biologicals NSB923 RPS6 [phospho Ser244/Ser247] Antibody 0.1ml Novus-Biologicals NSB922 RPS6 [phospho Ser235/Ser236] Antibody 0.1ml Novus-Biologicals NSB921 RPS6 [phospho Ser236] Antibody 0.1ml Novus-Biologicals NSB918 S6K [phospho Thr229] Antibody 0.1ml Novus-Biologicals NSB912 Src [phospho Tyr522] Antibody 0.1ml Novus-Biologicals NSB910 Hck [phospho Tyr207/Ser211] Antibody 0.1ml Novus-Biologicals NSB909 DAB1 [phospho Ser491] Antibody 0.1ml Novus-Biologicals NSB908 DAB1 [phospho Tyr220] Antibody 0.1ml Novus-Biologicals NSB906 DAB1 [phospho Tyr198] Antibody 0.1ml Novus-Biologicals NSB896 Met (c-Met) [phospho Tyr1365] Antibody 0.1ml Novus-Biologicals NSB892 Met (c-Met) [phospho Tyr1349] Antibody 0.1ml