Immunogen	Functions as a p65 decoy through phosphorylation of the Ser529/536 sites on the peptide.
Modification	p Ser529, p Ser536
Specificity	The NF-kB p65(Ser529/536) inhibitory peptide also contains a protein transduction (PTD) sequence(DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.The control peptide consists of only the PTD sequence.
PreparationMethod	Preparation of 5 mM Stock SolutionsPBS* is added directly to the vials to prepare the stock solutions.Note: Bring the solution to room temperature and quick spin the tubes before opening the caps. NF-kB p65(Ser529/536) Inhibitor Peptide: 1mg of DRQIKIWFQNRRMKWKKNGLLSGDEDFSS Add 54.1 ul PBS* to the vial, to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.Control Peptide: 1 mg of DRQIKIWFQNRRMKWKKAdd 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing. *Recipe for 1X PBS: 1. Dissolve the following in 800ml distilled H2O.- 8g of NaCl- 0.2g of KCl- 1.44g of Na2HPO4- 0.24g of KH2PO42. Adjust pH to 7.5 with HCl.3. Adjust volume to 1L with additional distilled H2O. 4. Sterilize by autoclaving
Content	Inhibitor peptide (NBP2-31224): 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKNGLLSGDEDFSS (p65 sequence: NGLLSGDEDFSS). Molecular weight: 3697.21Antennapedia Control peptide (NBP2-29334): 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
Gene	RELA

Application Notes

Inhibition of NF-kB activitiy. Researchers can study the effect of p65 inhibitor peptide using a variety of methods.Following is a general protocol for KBM-5 cells. It may need to be optimized for different cell types.Preincubate cells with appropriate amounts of inhibitory or control peptides for 1 hr and then treat with TNF or other NF-kB activating agents. Prepare nuclear extracts and check for the presence of NF-kB DNAbinding activity by EMSA (Figure 1). Nuclear extracts can be prepared as described by Bharati A, et al, 2003 and Takada et al, 2004 or using nuclear extraction kit.Please refer to Takada et al, 2004 for further details on the use of this inhibitory peptide.

Publications

ReadPublications using NBP2-29321 in the following applications: B/N 1 publication In vitro 1 publication

Novus Biologicals 美国Novus Biologicals公司是一家抗体专业公司，生产用于研究的单克隆和多克隆抗体，包含5万多种一抗和近1000种二抗，产品涉及神经生物学，细胞凋亡，肿瘤，DNA修复，干细胞标记，信号转导等多个研究方向。多年的抗体生产服务经验，使novus得以引领生物科学发展趋势，并可随时为生物科研工作者提供最新和最广泛的产品。Novus的愿景是通过开发和销售在前沿科学的独特产品来推动科学发现。Novus组织了商业化机制，致力于为生物研究界提供独特的生物材料。Novus还持续关注科学趋势，并为新的研究提供必要的材料。Novus的产品广泛使用于机构和商业研究，并在推动生物学研究中起着重要的。 Novus Biologicals is a biotech company based in Littleton, Colorado, USA with offices in San Diego, California, Oakville, ON, Canada and the Cambridge Science Park in Cambridge, UK.[1] The company develops, licenses and markets research materials to scientists in academic, corporate and government laboratories. Novus, like many others companies such as Abcam, Covance, Proteintech Group, EnCor Biotechnology and Millipore Corporation, arose to answer a call for research grade antibodies for the scientific market.Founded by Karen Padgett in 1996, Novus Biologicals has sought to partner with leading scientific researchers and third-party providers to develop an extensive range of more than 180,000 products.[2] The main products are antibodies, proteins, peptides, RNAi, cell lysates, isotype controls, slides, and kits. Padgett is company president and CEO, and has been active both in the academic and local Colorado communities. In 2008, Novus became a limited liability company when Mainsail Partners invested in the company.[4] Novus Biologicals has reported double digit revenue growth every year since its inception, has been selected six years in a row to the Inc. (magazine) 5000 fastest-growing private companies in America,[5] and was on the 2009 list of Colorado Companies to Watch.[6]In 2014 major antibody and reagent supplier Bio-Techne, acquired Novus Biologicals for $60 million.[7] The acquisition was meant to provide scientists and doctors a broader selection of products in one location. 产品列表： Novus-Biologicals SP4385P MC1 Receptor Antibody 0.05mg Novus-Biologicals PA184X Defensin beta 3 Protein 0.02mg Novus-Biologicals NSB998 PKA 2 beta [phospho Ser114] Antibody 0.1ml Novus-Biologicals NSB992 cAMP Protein Kinase Catalytic subunit beta [phospho Ser338] Antibody 0.05ml Novus-Biologicals NSB988 cAMP Protein Kinase Catalytic subunit alpha [phospho Thr197] Antibody 0.1ml Novus-Biologicals NSB979 PKC theta [phospho Ser695] Antibody 0.1ml Novus-Biologicals NSB978 PKC eta [phospho Ser674] Antibody 0.1ml Novus-Biologicals NSB977 PKC epsilon [phospho Ser729] Antibody 0.1ml Novus-Biologicals NSB976 PKC delta [phospho Ser664] Antibody 0.1ml Novus-Biologicals NSB975 PKC gamma [phospho Thr674] Antibody 0.1ml Novus-Biologicals NSB970 PKC theta [phospho Ser676] Antibody 0.1ml Novus-Biologicals NSB969 PKC eta [phospho Thr655] Antibody 0.1ml Novus-Biologicals NSB968 PKC iota [phospho Thr555/Thr563] Antibody 0.1ml Novus-Biologicals NSB966 PKC delta [phospho Ser645] Antibody 0.1ml Novus-Biologicals NSB965 PKC gamma [phospho Thr655] Antibody 0.1ml Novus-Biologicals NSB964 PKC beta [phospho Thr641] Antibody 0.1ml Novus-Biologicals NSB963 PKC beta [phospho Thr642] Antibody 0.1ml Novus-Biologicals NSB962 PKC alpha [phospho Thr638] Antibody 0.1ml Novus-Biologicals NSB961 PKC mu [phospho Ser738/Ser742] Antibody 0.1ml Novus-Biologicals NSB960 PKC theta [phospho Thr538] Antibody 0.1ml Novus-Biologicals NSB956 PKC gamma [phospho Thr514] Antibody 0.1ml Novus-Biologicals NSB955 PKC beta [phospho Thr500] Antibody 0.1ml Novus-Biologicals NSB948 GATA4 [phospho Ser105] Antibody 0.1ml Novus-Biologicals NSB944 NFAT1 [phospho Ser54] Antibody 0.1ml Novus-Biologicals NSB942 PAK1/2/3 [phospho Thr423] Antibody 0.1ml Novus-Biologicals NSB940 PAK1/2/3 [phospho Ser141] Antibody 0.1ml Novus-Biologicals NSB928 RSK1 [phospho Ser380] Antibody 0.1ml Novus-Biologicals NSB926 RSK1 [phospho Ser363] Antibody 0.1ml Novus-Biologicals NSB924 RSK1 [phospho Ser221] Antibody 0.1ml Novus-Biologicals NSB923 RPS6 [phospho Ser244/Ser247] Antibody 0.1ml Novus-Biologicals NSB922 RPS6 [phospho Ser235/Ser236] Antibody 0.1ml Novus-Biologicals NSB921 RPS6 [phospho Ser236] Antibody 0.1ml Novus-Biologicals NSB918 S6K [phospho Thr229] Antibody 0.1ml Novus-Biologicals NSB912 Src [phospho Tyr522] Antibody 0.1ml Novus-Biologicals NSB910 Hck [phospho Tyr207/Ser211] Antibody 0.1ml Novus-Biologicals NSB909 DAB1 [phospho Ser491] Antibody 0.1ml Novus-Biologicals NSB908 DAB1 [phospho Tyr220] Antibody 0.1ml Novus-Biologicals NSB906 DAB1 [phospho Tyr198] Antibody 0.1ml Novus-Biologicals NSB896 Met (c-Met) [phospho Tyr1365] Antibody 0.1ml Novus-Biologicals NSB892 Met (c-Met) [phospho Tyr1349] Antibody 0.1ml