Description	Tissue Lane Tissue Type Cat. No. Grade Stage Sex Age Diagnosis MWM 2 Lung NBP2-27810 2 II F 51 Adenocarcinoma 3 Lung NBP2-27808 Normal adjacent 4 Lung NBP2-27815 2 IB F 56 Adenocarcinoma 5 Lung NBP2-27813 Normal adjacent 6 Lung NBP2-27820 2 II F 63 Adenocarcinoma 7 Lung NBP2-27818 Normal adjacent 8 Lung NBP2-27825 2 II F 66 Adenocarcinoma 9 Lung NBP2-27823 Normal adjacent 10 Lung NBP2-27830 2 II F 53  Adenocarcinoma 11 Lung NBP2-27828 Normal adjacent 12 Lung NBP2-27840. 2 II F 32 Adenocarcinoma 13 Lung NBP2-27838 Normal adjacent 14 Lung NBP2-27845 2 IB F 59 Adenocarcinoma 15 Lung NBP2-27843 Normal adjacent
Background	The development of biomarkers has enormous potential for revolutionizing the diagnosis and treatment of disease. Western blot analysis is an integral technique for biomarker profiling. While key for biomarker discovery, the existing body of western blot data has primarily been defined from studies of tumor, immortal, and primary cells growing in vitro. Collectively, results obtained over dec...ades have been integral to the dogma that up- and down-regulation of proteins can be leveraged as biomarkers of normal development, homeostasis, and disease. However, data from human tissue-derived products is key for generating biomarker validation data and progressing the most promising biomarker targets towards the clinic. The OncoPair INSTA-Blot&8286; product line addresses the increasing demand for the inclusion of human tissue-derived products in biomarker profiling.The OncoPair INSTA-Blot&8286; is a ready-to-use PVDF western blot membrane containing denatured protein lysates manufactured from Novus extensive Human Clinical Tissue Lysate collection. Each blot contains 14 lanes of alternating tumor (T) and matched normal adjacent (NA) tissue lysates from 7 patient donors. Incorporating both T and NA on the blot enables protein expression analysis of the tumor and microenvironment. The tumor microenvironment is increasingly being recognized as a major factor in influencing malignant progression and metastatic potential.Cancer is a heterogeneous disease and the presence of multiple donors on a single blot enables rapid screening of protein expression variability between T/NA from different individuals and at different disease stages. OncoPair INSTA-Blots&8286; are available for a wide range of tumor types and stages. The product line is manufactured from donor patient tissue obtained from established hospital-based collection sites around the world. Tissues are collected under strict bioethical standards using IRB and HIPAA approved protocols. These protocols ensure patient confidentiality, safety, and informed consent. Tissues are flash frozen at collection sites within minutes of removal, maintained in liquid nitrogen and processed into lysates using protocols optimized for extracting proteins from tissues. Lysates are denatured for OncoPair INSTA-Blot&8286; production; native lysates are available for follow up proteomic studies. OncoPair INSTA-Blots&8286; and lysates are also useful in conjunction with the Cell Line and Normal Tissue INSTA-Blots&8286; and lysates for comprehensive antibody validation and to profile antibody reactivity and protein expression patterns across species and between tissues and cells lines. The OncoPair INSTA-Blots&8286; are proteomic discovery tools and are useful for screening the expression of various proteins in tumor and normal adjacent tissues from various donors. Researchers may want to follow up on their results by further analysis with the corresponding individual lysates when available. Show More
PreparationMethod	Tissue specimens were homogenized in modified RIPA buffer to obtain the soluble proteins and centrifuged to clarify. The concentration of protein in each lysate was determined by a standard protein assay and standardized to 1 mg/ml. Sample buffer was added to the soluble fraction and approximately 14 ug/lane of protein was run and then transferred to PVDF membrane.Soluble fraction extraction buffer (extract 1): PBS at pH 7.4, 1 ug/ml Aprotinin, 1 mM NaF -Modified RIPA buffer: 1 mM EDTA, 1 ug/ml Pepstatin-A, 0.1% SDS, 0.25% Na deoxycholate, 1 ug/ml Leupeptin, 1 mM PMSF, 1 mM Na3VO45X Sample buffer: 50 ml glycerol, 15 g SDS, 3.819 g Tris, pH 6.8, 25 ml 2-ME, 100 mg bromophenol blue, final volume of 100 ml with DI H2O.

Application Notes	Use in Western blot reported in scientific literature (PMID 25985210)
Publications	ReadPublications using NBP2-30123 in the following applications: WB 3 publications

Novus Biologicals 美国Novus Biologicals公司是一家抗体专业公司，生产用于研究的单克隆和多克隆抗体，包含5万多种一抗和近1000种二抗，产品涉及神经生物学，细胞凋亡，肿瘤，DNA修复，干细胞标记，信号转导等多个研究方向。多年的抗体生产服务经验，使novus得以引领生物科学发展趋势，并可随时为生物科研工作者提供最新和最广泛的产品。Novus的愿景是通过开发和销售在前沿科学的独特产品来推动科学发现。Novus组织了商业化机制，致力于为生物研究界提供独特的生物材料。Novus还持续关注科学趋势，并为新的研究提供必要的材料。Novus的产品广泛使用于机构和商业研究，并在推动生物学研究中起着重要的。 Novus Biologicals is a biotech company based in Littleton, Colorado, USA with offices in San Diego, California, Oakville, ON, Canada and the Cambridge Science Park in Cambridge, UK.[1] The company develops, licenses and markets research materials to scientists in academic, corporate and government laboratories. Novus, like many others companies such as Abcam, Covance, Proteintech Group, EnCor Biotechnology and Millipore Corporation, arose to answer a call for research grade antibodies for the scientific market.Founded by Karen Padgett in 1996, Novus Biologicals has sought to partner with leading scientific researchers and third-party providers to develop an extensive range of more than 180,000 products.[2] The main products are antibodies, proteins, peptides, RNAi, cell lysates, isotype controls, slides, and kits. Padgett is company president and CEO, and has been active both in the academic and local Colorado communities. In 2008, Novus became a limited liability company when Mainsail Partners invested in the company.[4] Novus Biologicals has reported double digit revenue growth every year since its inception, has been selected six years in a row to the Inc. (magazine) 5000 fastest-growing private companies in America,[5] and was on the 2009 list of Colorado Companies to Watch.[6]In 2014 major antibody and reagent supplier Bio-Techne, acquired Novus Biologicals for $60 million.[7] The acquisition was meant to provide scientists and doctors a broader selection of products in one location. 产品列表： Novus-Biologicals SP4385P MC1 Receptor Antibody 0.05mg Novus-Biologicals PA184X Defensin beta 3 Protein 0.02mg Novus-Biologicals NSB998 PKA 2 beta [phospho Ser114] Antibody 0.1ml Novus-Biologicals NSB992 cAMP Protein Kinase Catalytic subunit beta [phospho Ser338] Antibody 0.05ml Novus-Biologicals NSB988 cAMP Protein Kinase Catalytic subunit alpha [phospho Thr197] Antibody 0.1ml Novus-Biologicals NSB979 PKC theta [phospho Ser695] Antibody 0.1ml Novus-Biologicals NSB978 PKC eta [phospho Ser674] Antibody 0.1ml Novus-Biologicals NSB977 PKC epsilon [phospho Ser729] Antibody 0.1ml Novus-Biologicals NSB976 PKC delta [phospho Ser664] Antibody 0.1ml Novus-Biologicals NSB975 PKC gamma [phospho Thr674] Antibody 0.1ml Novus-Biologicals NSB970 PKC theta [phospho Ser676] Antibody 0.1ml Novus-Biologicals NSB969 PKC eta [phospho Thr655] Antibody 0.1ml Novus-Biologicals NSB968 PKC iota [phospho Thr555/Thr563] Antibody 0.1ml Novus-Biologicals NSB966 PKC delta [phospho Ser645] Antibody 0.1ml Novus-Biologicals NSB965 PKC gamma [phospho Thr655] Antibody 0.1ml Novus-Biologicals NSB964 PKC beta [phospho Thr641] Antibody 0.1ml Novus-Biologicals NSB963 PKC beta [phospho Thr642] Antibody 0.1ml Novus-Biologicals NSB962 PKC alpha [phospho Thr638] Antibody 0.1ml Novus-Biologicals NSB961 PKC mu [phospho Ser738/Ser742] Antibody 0.1ml Novus-Biologicals NSB960 PKC theta [phospho Thr538] Antibody 0.1ml Novus-Biologicals NSB956 PKC gamma [phospho Thr514] Antibody 0.1ml Novus-Biologicals NSB955 PKC beta [phospho Thr500] Antibody 0.1ml Novus-Biologicals NSB948 GATA4 [phospho Ser105] Antibody 0.1ml Novus-Biologicals NSB944 NFAT1 [phospho Ser54] Antibody 0.1ml Novus-Biologicals NSB942 PAK1/2/3 [phospho Thr423] Antibody 0.1ml Novus-Biologicals NSB940 PAK1/2/3 [phospho Ser141] Antibody 0.1ml Novus-Biologicals NSB928 RSK1 [phospho Ser380] Antibody 0.1ml Novus-Biologicals NSB926 RSK1 [phospho Ser363] Antibody 0.1ml Novus-Biologicals NSB924 RSK1 [phospho Ser221] Antibody 0.1ml Novus-Biologicals NSB923 RPS6 [phospho Ser244/Ser247] Antibody 0.1ml Novus-Biologicals NSB922 RPS6 [phospho Ser235/Ser236] Antibody 0.1ml Novus-Biologicals NSB921 RPS6 [phospho Ser236] Antibody 0.1ml Novus-Biologicals NSB918 S6K [phospho Thr229] Antibody 0.1ml Novus-Biologicals NSB912 Src [phospho Tyr522] Antibody 0.1ml Novus-Biologicals NSB910 Hck [phospho Tyr207/Ser211] Antibody 0.1ml Novus-Biologicals NSB909 DAB1 [phospho Ser491] Antibody 0.1ml Novus-Biologicals NSB908 DAB1 [phospho Tyr220] Antibody 0.1ml Novus-Biologicals NSB906 DAB1 [phospho Tyr198] Antibody 0.1ml Novus-Biologicals NSB896 Met (c-Met) [phospho Tyr1365] Antibody 0.1ml Novus-Biologicals NSB892 Met (c-Met) [phospho Tyr1349] Antibody 0.1ml