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蚂蚁淘在线 / 品牌中心 / Epigentek / Epigentek/EpiQuik全局乙酰组蛋白H3K18定量试剂盒（比色法）/P-4014-96/96分析

Epigentek/EpiQuik全局乙酰组蛋白H3K18定量试剂盒（比色法）/P-4014-96/96分析

价格
￥10180.00
货号：P-4014-96
浏览量：127
品牌：Epigentek
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商品描述
InputType:HistoneExtracts
ResearchArea:HistoneAcetylation
TargetApplication:AmountQuantitation
VesselFormat:96-WellPlate
100%Guarantee:6months
ProductOverview

The EpiQuik™GlobalAcetylHistoneH3-K18QuantificationKit(Colorimetric) isaconvenientpackageoftoolsthatallowstheexperimentertospecificallymeasureglobalacetylationofhistoneH3-K18 colorimetrically,usingavarietyofmammaliancells(human,mouse,etc.)includingfreshandfrozentissues,andculturedadherentandsUSPensioncells. Thekithasthefollowingadvantages:

  • Quickandefficientprocedure,whichcanbefinishedwithin2.5hours.
  • InnovativecolorimetricassaywithouttheneedforrADIoactivity,electrophoresis,orchromatography.
  • SpecificallycapturesacetylH3-K18withthedetectionlimitaslowas2ng/wellanddetectionrangefrom20ng-5µg/wellofhistoneextracts.
  • ThecontrolisconvenientlyincludedforthequantificationoftheamountofacetylH3-K18.
  • StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughput.
  • Simple,reliable,andconsistentassayconditions.

BackgroundInformation
Acetylationofhistones,includinghistoneH3,hasbeeninvolvedintheregulationofchromatinstructureandrecruitmentoftranscriptionfactorstothegenepromoters.Histoneacetyltransferases(HATs)andhistonedeacetylases(HDACs)playacriticalroleincontrolofhistoneH3acetylationatmultiplesites.HistoneH3atlysine18(H3-K18),alongwithK9andK14,areprimaryacetylatedsitesofhistoneH3.AcetylationofhistoneH3-K18istightlyinvolvedinthecellcyclereguation,cellproliferationandapoptosis.AcetylationofhistoneH3-K18isalsocorrelatedwithtranscriptionactivation.AnimbalanceintheequilibriumofhistoneH3acetylation,includingK18acetylation,hasbeenassociatedwithtumOrigenesisandcancerprogression.HistoneH3-K18acetylationmaybeincreasedbyinhibitionofHDACsanddecreasedbyHATinhibition.Thus,quantitativedetectionofglobalacetylhistoneH3-K18wouldprovideusefulinformationforbetterunderstandingepigeneticregulationofgeneactivation,andfordevelopingHATorHDAC-targeteddrugs.TheEpiQuik™GlobalAcetylHistoneH3-K18QuantificationKit(Colorimetric)providesatoolformeasuringglobalacetylationofhistoneH3-K18.

Principle&Procedure
Thiskit isdesignedformeasuringglobalhistoneH3-K18acetylation.Inanassaywiththiskit,theacetylhistoneH3atlysine18iscapturedtothestripwellscoatedwithananti-acetylH3-K18antibody.ThecapturedacetylhistoneH3-K18canthenbedetectedwithalabeleddetectionantibody,followedbyacolordevelopmentreagent.TheratioofacetylH3-K18isproportionaltotheintensityofabsorbance.TheabsoluteamountofacetylH3-K18canbequantifiedbycomparingtothestandardcontrol.

StartingMaterials
Inputmaterialshouldbepurifiedhistoneextracts.Ingeneral,theinputamountshouldbefrom50ngto200ngperwellofhistoneextracts.

 

ProductComponents

C1(10Xwashbuffer)
C2(antibodybuffer)
C3(detectionantibody,1mg/ml)*
C4(colordeveloper)
C5(stopsolution)
Standardcontrol(100µg/ml)*
8-Wellsamplestrips(withframe)
8-Wellstandardcontrolstrips
UserGuide

*Formaximumrecoveryoftheproducts,centrifugetheoriginalvialpriortoopeningthecap.

Epigentek已经覆盖了您。使用我们任何一种便捷的ChIP套件都能满足您的研究需求。Epigentek于2005年首次推出了基于微孔板的ChIP格式，从而为表观遗传研究人员提供了快速低成本的染色质样品免疫沉淀方法。每个试剂盒均包含您所需的一切，以快速有效的方式可靠地免疫沉淀染色质。