AntibodiesOnline/CA12 (Extracellular Domain), (AA 1291) (Active) protein (His tag)/ABIN2003147/20

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ProteinName

CA12

• CAXII
• HsT18816
• CAR12
• 2310047E01Rik
• AI314958
• Ca12
• carbonicanhydraseXII
• carbonicanyhydrase12
• CA12
• Car12

ProteinCharacteristics

ExtracellularDomain,AA1-291

3AA25-301

2AA25-354

1AA1-301

1AA1-354

1AA154-307

1AA161-318

1AA25-124

1AA25-303

1ExtracellularDomain

1TranscriptVariant1

1fulllength

1partial

Origin

Human

7Human

6Mouse(Murine)

2Rat(Rattus)

1Rabbit

Source

HumanCells

8Escherichiacoli(E.coli)

2InsectCells

2Wheatgerm

1HEK-293Cells

1HumanCells

1MammalianCells

1Yeast

ProteinType

Recombinant

BiologicalActivity

Active

Purificationtag/Conjugate

Histag

Application

FunctionalStudies(Func),SDS-PAGE(SDS)

Options

Bulkdiscount

Supplier

SupplierProductNo.

Productdetails ReferencesImagesHandlingApplicationDetailsTargetdetailsbacktotop
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Characteristics	TherecombinanthumanCA12consistingof278aminoacidsandhasacalculatedmolecularmassof31.6kDa.Asaresultofglycosylation,therecombinantproteinmigratesasanapproximately40-45kDaproteininSDS-PAGEunderreducingconditions. ProteinStructure:ADNAsequenceencodingtheextracellulardomainofhumanCA12(NP_001209.1)(Met1-Gln291)wasexpressedwithaC-terminalpolyhistidinetag. PredictedN-Term:Ala25
Purity	>97 %asdeterminedbySDS-PAGE
EndotoxinLevel	<1.0EUperμgoftheproteinasdeterminedbytheLALmethod
ProductDetails:BiologicalActivityComment	Measuredbyitsesteraseactivity.Thespecificactivityis>40pmoles/min/μg,asmeasuredwith1mM4-Nitrophenylacetateand2.5μgenzymeat400nmin100μLof12.5mMTris,75mMNaCl,pH7.5.

Targetdetails ReferencesImagesHandlingApplicationDetailsbacktotop
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Background	Synonyms:CA12,CAXII,FLJ20151,HsT18816
MolecularWeight	31.6kDa,40-45kDa
NCBIAccession	NP_001209
ResearchArea	Cancer

ApplicationDetails ReferencesImagesHandlingTargetdetailsbacktotop
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ApplicationNotes	Optimalworkingdilutionshouldbedeterminedbytheinvestigator.
Restrictions	ForResearchUseonly

Handling ReferencesImagesApplicationDetailsTargetdetailsbacktotop
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Format	Lyophilized
Buffer	LyophilizedfromsterilePBS, pH7.4 Normally5 %-8 %trehalose,mannitoland0.01 %Tween80areaddedasprotectantsbeforelyophilization.
HandlingAdvice	Avoidrepeatedfreeze-thawcycles.Itisrecommendedthattheproteinbealiquotedforoptimalstorage.
Storage	-20°C,-80°C
StorageComment	Storeitundersterileconditionsat-20°Cto-80°C.Itisrecommendedthattheproteinbealiquotedforoptimalstorage.Avoidrepeatedfreeze-thawcycles. Shipping:Ingeneral,recombinantproteinsareprovidedaslyophilizedpowderwhichareshippedatambienttemperature. Bulkpackagesofrecombinantproteinsareprovidedasfrozenliquid.Theyareshippedoutwithblueiceunlesscustomersrequireotherwise.
ExpiryDate	12months

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SupplierImages	CA12(ExtracellularDomain),(AA1-291)(Active)protein(Histag) ×CA12(ExtracellularDomain),(AA1-291)(Active)protein(Histag) CA12(ExtracellularDomain),(AA1-291)(Active)protein(Histag) Close

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Productcitedin:	Lecoeur,Goossens,Vaccher,Bonte,Foulon:"Amultivariateapproachforthedeterminationofisoelectricpointofhumancarbonicanhydraseisoformsbycapillaryisoelectricfocusing."in:Electrophoresis,Vol.32,Issue20,pp.2857-66,2011(PubMed).
Backgroundpublications	Ivanov,Kuzmin,Wei,Pack,Geil,Johnson,Stanbridge,Lerman:"Down-regulationoftransmembranecarbonicanhydrasesinrenalcellcarcinomacelllinesbywild-typevonHippel-Lindautransgenes."in:ProceedingsoftheNationalAcademyofSciencesoftheUnitedStatesofAmerica,Vol.95,Issue21,pp.12596-601,1998(PubMed).

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