RACE试剂盒
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ProductSpecification:
| AlternativeName: | TransientreceptorpotentialchannelsubfamilyMmember8 |
| Host: | Rabbit |
| Immunogen: | Syntheticpeptidecorrespondingtoaa917-929locatedinthethirdextracellularloopofhumanTRPM8. |
| UniProtID: | Q7Z2W7 |
| Speciesreactivity: | Human,Mouse,Rat |
| Applications: | ICC,IP,WB |
| BlockingPeptide: | Included:TRPM8ControlPeptide(40µg).Suppliedlyophilized. |
| PurityDetail: | AffinitypurifiedIgG. |
| Formulation: | LyophilizedfromPBS,pH7.4,1%BSAand0.05%sodiumazide. |
| Reconstitution: | Tolyophilizedantibody,add50µlor200µl(dependingonwhichsizewaspurchased)ofdeionizedwater.Tolyophilizedpeptide,add100µlofdeionizedwater. |
| Use/Stability: | Lyophilizedantibodyandlyophilizedpeptidecanbestoredintactatroomtemperatureforseveralweeks.Forlongerperiodstheyshouldbestoredat-20°C.Afterreconstitution,theantibodysolutioncanbestoredat4°Cforupto2weeks.Forlongerperiods,smallaliquotsshouldbestoredat-20°Corbelow.Centrifugeallantibodypreparationsbeforeuse(10,000Xgfor5minutes).Afterreconstitution,controlpeptideshouldbestoredat-20°C. |
| Shipping: | ShippedonBlueIce |
| LongTermStorage: | -20°C |
| ScientificBackground: | Themammalianmelastatin-relatedtransientreceptorpotential(TRPM)isasubfamilyoftheTRPfamily.Thefamilywasnamedafterthefirstmember,melastatin(TRPM1),whosegenewasidentifiedinmetastaticandbenignmelanomas.TheTRPMproteinssharestructuralhomologywithothermembersoftheTRPsuperfamilychannels;sixputativetransmembranedomains,andcytoplasmicN-terminusandC-terminus.However,duetotheirlongN-terminusandC-terminustheywerealsonamedthelongTRPchannelfamily.TRPM8isthecoldandmentholreceptoractivatedbyeithercoldtemperatures(~28°C)ormenthol. |
| TechnicalInfo/ProductNotes: | PREADSORPTIONCONTROL:1µgpeptideper1µgantibody.Prepare2tubes.Inthefirst,dilutethenecessaryamountofantibodyin200-500µlofPBS,1%BSAand0.025%sodiumazide.Preparethesecondtubeidenticallytothefirstwiththeadditionoftheappropriateamountofpeptide.Incubateatroomtemperaturefor1hr.Centrifugebothtubesat10,000Xgfor5min.Usethesupernatantsfromtubes1and2forparallelexperiments. |
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