Platecharacteristics:

Contents:

• Coatingantibodies
• Biotinylateddetectorantibodies
• Phi-labeledgoatanti-biotinantibodies
• Activatorsolutions
• Blockingstocksolution
• DilutionbufferT
• Tween-20
• TransparentELISPOTplateswithlidsandcoverslips
• Detailedmanual

Referenceslist:

• ComparisonofcryopreservationmethodsonT-cellresponsestoisletandcontrolantigensfromtype1diabeticpatientsandcontrols.Brooks-Worrell,B,etal.DiabetesMetab.Res.Rev.2011Nov;27:737-45
• Self-glycolipidsmodulatedendriticcellschangingthecytokineprofilesofcommittedautoreactiveTcells.Buschard,Karsten,etal.PLoSONE2012;7:e52639
• Donor-reactivecytokineprofilesafterHLA-identicalliving-relatedkidneytransplantation.Gerrits,JeroenH,etal.Nephrol.Dial.Transplant.2008Jun;23:2016-23
• AnalysisofT-cellresponsesinmetastaticmelanomapatientsvaccinatedwithdendriticcellspulsedwithtumorlysates.Griffioen,Marieke,etal.CancerImmunol.Immunother.2004Aug;53:715-22
• Developmentoftype1diabetesdespiteseverehereditaryB-lymphocytedeficiency.Martin,S,etal.N.Engl.J.Med.2001Oct4;345:1036-40
• AssociationofT-cellreactivitywithbeta-cellfunctioninrecentonsettype1diabetespatients.Pfleger,Christian,etal.J.Autoimmun.2010Mar;34:127-35
• Beta-cellfunctioninnew-onsettype1diabetesandimmunomodulationwithaheat-shockproteinpeptide(DiaPep277):arandomised,double-blind,phaseIItrial.Raz,I,etal.Lancet2001Nov24;358:1749-53
• ComparisonofcytokineELISpotassayformatsforthedetectionofisletantigenautoreactiveTcells.ReportofthethirdimmunologyofdiabetessocietyT-cellworkshop.Schloot,NanetteC,etal.J.Autoimmun.2003Dec;21:365-76
• Analysisofanthraxandplaguebiowarfarevaccineinteractionswithhumanmonocyte-deriveddendriticcells.Skowera,Anna,etal.J.Immunol.2005Dec1;175:7235-43
• Cellularinterferon-γandinterleukin-13immunereactivityintype1,type2andlatentautoimmunediabetes:actionLADA6.Strom,Alexander,etal.Cytokine2012May;58:148-51
• RedirectionofhumanautoreactiveT-cellsUponinteractionwithdendriticcellsmodulatedbyTX527,ananalogof1,25dihydroxyvitaminD(3).vanHalteren,AstridGS,etal.Diabetes2002Jul;51:2119-25