货号：	EMM1115-1
样本：	50μl
应用：	血清、血浆、细胞培养上清液、尿液
检测方法：	ELISA
检测限：	0.07ng/ml
供应商：	AssayPro
数量：	大量
规格：	96wells

Introduction
Alpha-2-Macroglobulinisamajorserumproteinwithdiversefunctions,includinginhibitionofproteaseactivityandbindingofgrowthfactors,cytokines,anddiseasefactors(1).Increasedserumα2-macroglobulinhasbeensuggestedtobeassociatedwithmultiplesclerosis(MS)(2),glomerulardisease(3),andliverdiseases(4).Mouseα-macroglobulin(MouseAMG)isbelievedtobeafunctionalhomologueofhumanα2-macroglobulin(Humanα2M).
 
PrincipleoftheAssay
TheAssayMaxMouseα-MacroglobulinELISAkitisdesignedfordetectionofmouseα-macroglobulininmouseplasma,serum,urine,andcellculturesupernatants.Thisassayemploysaquantitativesandwichenzymeimmunoassaytechniquethatmeasuresmouseα-Macroglobulininlessthan4hours.Apolyclonalantibodyspecificforα-Macroglobulinhasbeenpre-coatedontoamicroplate.Alpha-macroglobulininstandardsandsamplesissandwichedbytheimmobilizedantibodyandabiotinylatedpolyclonalantibodyspecificforα-macroglobulin,whichisrecognizedbyastreptavidin-peroxidaseconjugate.Allunboundmaterialisthenwashedawayandaperoxidaseenzymesubstrateisadded.Thecolordevelopmentisstoppedandtheintensityofthecolorismeasured.