货号：	klRPA792Hu01
供应商：	柯雷生物
数量：	大量
英文名：	RecombinantTopoisomeraseII(TOP2)
保存条件：	负20度

[PROPERTIES]
Residues:Ile923~Arg1148
Tags:TwoN-terminalTags,His-tagandT7-tag
Accession:P11388
Host:E.coli
SubcellularLocation:Cytoplasm.Nucleus,nucleoplasm.
Purity:>95%
EndotoxinLevel:<1.0EUper1μg(determinedbytheLALmethod).
Formulation:Suppliedaslyophilizedformin20mMTris,150mMNaCl,pH8.0,containing1mMEDTA,1mMDTT,0.01%sarcosyl,5%trehalose,andpreservative.Predictedisoelectricpoint:6.2PredictedMolecularMass:30.3kDa
Applications:SDS-PAGE;WB;ELISA;IP.(Maybesuitableforuseinotherassaystobedeterminedbytheenduser.)
[USAGE]
ReconstituteinsterileddH2O
[STORAGEANDSTABILITY]
Storage:Avoidrepeatedfreeze/thawcycles.Storeat2-8oCforonemonth.Aliquotandstoreat-80oCfor12months.StabilityTest:Thethermalstabilityisdescribedbythelossrateofthetargetprotein.Thelossratewasdeterminedbyacceleratedthermaldegradationtest,thatis,incubatetheproteinat37oCfor48h,andnoobviousdegradationandprecipitationwereobserved.
(ReferringfromChinaBIOLOGicalProductsStandard,whichwascalculatedbytheArrheniusequation.)Thelossofthisproteinislessthan5%withintheexpirationdateunderappropriatestoragecondition.
[SEQUENCES]
Thesequenceofthetargetproteinislistedbelow.
ISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLR[REFERENCES]1.Tsai-PflugfelderM.,etal.(1988)Proc.Natl.Acad.Sci.U.S.A.85:7177-7181.2.WassermanR.A.,etal.(1993)CancerRes.53:3591-3596.3.LangA.J.,etal.(1998)Gene221:255-266.4.SngJ.H.,etal.(1999)Biochim.Biophys.Acta1444:395-406