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productdatasheet

Anti-pan-SAPAPMonoclonalAntibody

ORDERINGINFORMATION

CatalogNo.:56464(cloneS127-31)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.

BACKGROUND

TheSAPAPproteinfamilyhasatleastfourmembersandundergoescomplexalternativesplicing.Theyarethoughttobeadaptorproteinsthatinteractwithdifferentsynapticscaffoldingproteins,cytoskeletal,andsignalingcomponents.SAPAPinteractswithGKdomainsofPSD-95andalsobindsShankattheC-terminalregion.SAPAPsappeartobeimportantinrecruitingandclusteringofNMDAreceptorsandMAGUK(Membrane-AssociatedGuanylateKinase)-basedsignalingcomplexes.

SPECIFICATIONSUMMARYAntigen:Fusionprotein,aa772-992(C-terminus)ofratSAPAP1(accessionno.P97836).HostSpecies:MouseAntibodyClass:IgG2b

SPECIFICITY

Thisantibodyrecognizeshuman,mouseandratSAPAP1,2,3,and4.

APPLICATIONS

Immunoblotting:useat1-10ug/ml.Bandsof~120kDa(SAPAP2)and~110kDa(SAPAP1/3/4)aredetected.

MBM=mousebrain,RBM=ratbrain,

1stHBM=humanhippocampus,2ndHBM=humancerebralcortex.

ImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/mlImmunofluorescence:useat1-10ug/mlThesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

Positivecontrol:Ratbrainlysate.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

QEDBioscience,Inc.10919TechnologyPlace,SuiteC

SanDiego,CA92127

TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com

Visitourwebsiteforadditionalproductinformationandtoorderonline.

qedbio.com

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