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RabbitAnti-beta-Actin(LoADIngControl) antibody

产品编号	hz-0061R
英文名称	beta-Actin(LoadingControl)
中文名称	β-肌动蛋白/β-Actin抗体（内参抗体）
别名	BetaActin;beta-Actin;ACTB;Actincytoplasmic1;Actin,beta;Betaactin;betacytoskeletalactin;AXactinlikeprotein;ACTB;Actincytoplasmic1;alphasarcomericActin;Actx;Betacytoskeletalactin;MelanomaXactin;PS1TP5BP1;ACTB_HUMAN.

产品类型	内参抗体
研究领域	肿瘤细胞生物信号转导细胞骨架
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep, Bee, Fish, GuineaPig, Hamster, Goat, Cat, mt,op
产品应用	WB=1:5000-20000 ELISA=1:5000-20000 IHC-P=1:500-1000 IHC-F=1:500-1000 Flow-Cyt=1μg/Test IF=1:100-1000 （石蜡切片需做抗原修复） notyettestedinotherapplications.optimaldilutions/concentrationsshouldbedeterminedbytheenduser.
分子量	42kDa
细胞定位	细胞浆
性状	LyophilizedorLiquid
浓度	1mg/ml
免疫原	SyntheticMAPpeptidederivedfromhumanbeta-Actin:1-200/375
亚型	IgG
纯化方法	affinitypurifiedbyProteinA
储存液	0.01MTBS(pH7.4)with1%BSA,0.03%Proclin300and50%Glycerol.
保存条件	Storeat-20°Cforoneyear.Avoidrepeatedfreeze/thawcycles.Thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20°C.WhenreconstitutedinsterilepH7.40.01MPBSordiluentofantibodytheantibodyisstableforatleasttwoweeksat2-4°C.
PubMed	PubMed
产品介绍	background:LoadingControlThisgeneencodesoneofsixdifferentactinproteins.Actinsarehighlyconservedproteinsthatareinvolvedincellmotility,structure,andintegrity.Thisactinisamajorconstituentofthecontractileapparatusandoneofthetwononmusclecytoskeletalactins.[providedbyRefSeq,Jul2008].Function:Actinsarehighlyconservedproteinsthatareinvolvedinvarioustypesofcellmotilityandareubiquitouslyexpressedinalleukaryoticcells.Subunit:Polymerizationofglobularactin(G-actin)leadstoastructuralfilament(F-actin)intheformofatwo-strandedhelix.Eachactincanbindto4others.IdentifiedinamRNPgranulecomplex,atleastcomposedofACTB,ACTN4,DHX9,ERG,HNRNPA1,HNRNPA2B1,HNRNPAB,HNRNPD,HNRNPL,HNRNPR,HNRNPU,HSPA1,HSPA8,IGF2BP1,ILF2,ILF3,NCBP1,NCL,PABPC1,PABPC4,PABPN1,RPLP0,RPS3,RPS3A,RPS4X,RPS8,RPS9,SYNCRIP,TROVE2,YBX1anduntranslatedmRNAs.ComponentoftheBAFcomplex,whichincludesatleastactin(ACTB),ARID1A,ARID1B/BAF250,SMARCA2,SMARCA4/BRG1,ACTL6A/BAF53,ACTL6B/BAF53B,SMARCE1/BAF57SMARCC1/BAF155,SMARCC2/BAF170,SMARCB1/SNF5/INI1,andoneormoreofSMARCD1/BAF60A,SMARCD2/BAF60B,orSMARCD3/BAF60C.Inmusclecells,theBAFcomplexalsocontainsDPF3.FoundinacomplexwithXPO6,Ran,ACTBandPFN1.ComponentoftheMLL5-Lcomplex,atleastcomposedofMLL5,STK38,PPP1CA,PPP1CB,PPP1CC,HCFC1,ACTBandOGT.InteractswithXPO6andEMD.InteractswithERBB2.SubcellularLocation:Cytoplasm.Cytoskeleton.TissueSpecificity:Ubiquitouslyexpressedinalleukaryoticcells.Post-translationalmodifications:ISGylated.OxidationofMet-44byMICALs(MICAL1,MICAL2orMICAL3)toformmethioninesulfoxidepromotesactinfilamentdepolymerization.Methioninesulfoxideisproducedstereospecifically,butitisnotknownwhetherthe(S)-S-oxideorthe(R)-S-oxideisproduced.DISEASE:DefectsinACTA1arethecauseofnemalinemyopathytype3(NEM3)[MIM:161800].Aformofnemalinemyopathy.Nemalinemyopathiesaremusculardisorderscharacterizedbymuscleweaknessofvaryingseverityandonset,andabnormalthread-orrod-likestructuresinmusclefibersonhistologicexamination.Thephenotypeathistologicallevelisvariable.Somepatientspresentareasdevoidofoxidativeactivitycontaing(cores)withinmyofibers.Corelesionsareunstructuredandpoorlycircumscribed.DefectsinACTA1areacauseofmyopathycongenitalwithexcessofthinmyofilaments(MPCETM)[MIM:161800].Acongenitalmusculardisordercharacterizedathistologicallevelbyareasofsarcoplasmdevoidofnormalmyofibrilsandmitochondria,andreplacedwithdensemassesofthinfilaments.Centralcores,rods,raggedredfibers,andnecrosisareabsent.Similarity:Belongstotheactinfamily.SWISS:P60709GeneID:60Databaselinks: EntrezGene:396526Chicken EntrezGene:60Human EntrezGene:11461Mouse EntrezGene:100009272Rabbit EntrezGene:81822Rat Omim:102630Human SwissProt:P60706Chicken SwissProt:P60712Cow SwissProt:P60708Horse SwissProt:P60709Human SwissProt:P60710Mouse SwissProt:P29751Rabbit SwissProt:P60711Rat SwissProt:P60713Sheep Unigene:520640Human Unigene:708120Human Unigene:727576Human Unigene:328431Mouse Unigene:391967Mouse Unigene:94978Rat ImportantNote:Thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications. 内参抗体β-Actin是横纹肌肌纤维中的一种主要蛋白质成分，也是肌肉细丝及细胞骨架微丝的主要成分。具有收缩功能，分布广泛，具有高度保守性，在细胞中的表达相对稳定，因此常被用作校正系统的内参。β-Actin分子量为42kDa，此抗体主要用于标记平滑肌及其来源的肿瘤。我公司开发的β-Actin抗体已被国内外广大科研工作者使用，被称谓：质量信得过产品.
产品图片	Sample: A549Cell(Human)Lysateat30ugPrimary:Lane1:Anti-beta-Actin(bs-0061R)at1/500dilutionLane2:Anti-beta-Actin(bs-0061R)at1/1000dilutionLane3:Anti-beta-Actin(bs-0061R)at1/2000dilutionLane4:Anti-beta-Actin(bs-0061R)at1/4000dilutionLane5:Anti-beta-Actin(bs-0061R)at1/5000dilutionLane6:Anti-beta-Actin(bs-0061R)at1/8000dilutionLane7:Anti-beta-Actin(bs-0061R)at1/10000dilutionSecondary:IRDye800CWGoatAnti-RabbitIgGat1/20000dilutionPredictedbandsize:42kDObservedbandsize:47kD Sample: A549Cell(Human)Lysateat30ugPrimary:Lane1:Anti-beta-Actin(bs-0061R)at1/500dilutionLane2:Anti-beta-Actin(bs-0061R)at1/1000dilutionLane3:Anti-beta-Actin(bs-0061R)at1/2000dilutionLane4:Anti-beta-Actin(bs-0061R)at1/4000dilutionLane5:Anti-beta-Actin(bs-0061R)at1/5000dilutionLane6:Anti-beta-Actin(bs-0061R)at1/8000dilutionLane7:Anti-beta-Actin(bs-0061R)at1/10000dilutionSecondary:IRDye800CWGoatAnti-RabbitIgGat1/20000dilutionPredictedbandsize:42kDObservedbandsize:47kD Sample: Lane1:293TCellLysateat25ugLane2:A549CellLysateat25ugLane3:A431CellLysateat25ugPrimary:Anti-beta-Actin(bs-0061R)at1/1000and1/5000dilutionSecondary:IRDye800CWGoatAnti-RabbitIgGat1/20000dilutionPredictedbandsize:42kDObservedbandsize:42kD Sample:Lunglysateat30ug;Primary:Anti-beta-actin(bs-0061R)at1:1000dilution Secondary:HRPconjugatedGoat-Anti-RabbitIgG(bse-0295G)at1:3000dilutionPredictedbandsize:42kDObservedbandsize:42kD Tissue/cell:humancervicalcarcinoma;4%Paraformaldehyde-fixedandparaffin-embedded; Antigenretrieval:citratebuffer(0.01M,pH6.0),Boilingbathingfor15min;Blockendogenousperoxidaseby3%Hydrogenperoxidefor30min;Blockingbuffer(normalgoatserum,C-0005)at37℃for20min; Incubation:Anti-Beta-actinPolyclonalAntibody,Unconjugated(bs-0061R)1:1500,overnightat4°C,followedbyconjugationtothesecondaryantibody(SP-0023)andDAB(C-0010)staining Blankcontrol:RSC96(blue). PrimaryAntibody:RabbitAnti-beta-Actin/FITCConjugatedantibody(bs-0061R/FITC),Dilution:1μgin100μL1XPBScontaining0.5%BSA; IsotypeControlAntibody:RabbitIgG/FITC(orange),usedunderthesameconditions.ProtocolThecellswerefixedwith2%paraformaldehyde(10min)andthenpermeABIlizedwithice-cold90%methanolfor30minonice.Thecellswerewashedtwicewith1XPBS.Thecellswereincubatedin1XPBScontaining0.5%BSA+10%goatserum(15min)toblocknon-specificprotein-proteininteractionsfollowedbytheincubatedwithantibody(bs-0061R/FITC,1μg/1x10^6cells)for30minonice.Acquisitionof20,000eventswasperformed

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