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TeloCol®-6TypeICollagenSolution,6mg/ml(Bovine)

Catalog#5225

TeloCol®-6is50mLof6mg/ml,typeIbovinetelocollagensolutionfor2Dcoatingsor3Dhydrogels.Thiskitcomeswithaneutralizationsolutionforeasyhandlingandpreparation.Beingahigherconcentrationtelocollagen,TeloCol®-6willformfirmhydrogels.

ProductDescription

TeloCol®-6bovinecollagensolutionisderivedfromanacidextractionprocessyieldingatelopeptide-intactcollagen.Thepro-peptideregionsatbothendsofthecollagenchain,N-andC-telopeptideregions,aremaintained.

TeloCol®-6collagenisapproximately95%TypeIcollagenwiththeremainderbeingcomprisedofTypeIIIcollagen.Eachproductincludesabottlecontaining50mlofcollagensolutionaccompaniedwithabottleofpre-formulatedneutralizingsolutionfortheformationofacollagengel.Thisproductissuppliedataconcentrationofapproximately6mg/ml.TeloCol®-6issterilefilteredandprovidedinuser-friendlypackagingforuseandstorage.

TeloCol®-6isidealforcoatingofsurfaces,providingpreparationofthinlayersforculturingcells,oruseasasolidgel.

Parameter,Testing,andMethod

TeloCol®-6TypeICollagen#5225

SterilizationMethod

Filtration

ExtractionMethod

Acid-telocollagen

Form

Solution

PackageSize

50mLKit

StorageTemperatureofCollagen

2-10°C

StorageTemperatureofNeutralizationSolution

RoomTemperature

ShelfLife

Minimumof6monthsfromdateofreceipt

CollagenConcentration-Biuret

5.8-6.8mg/mL

CollagenPurity-SilverStaining

99%

pH

1.9-3.0

KineticGelTest(Minutes)

40

GelFormationTubeTest(Minutes)

40

Fibrillogenesis(AbsorbanceUnits)

0.35

ElectrophoreticPattern-CoomassieBlue

Characteristic

Sterility-USPmodified

Nogrowth

Endotoxin-LAL

10.0EU/mL

Osmolality(mOsmoH2O/kg)

35

CellAttachmentAssay

Pass

Source

BovineHide

HydrogelYoungsModulusE(Pa)

Characteristic

ProductReferences

ReferencesforTeloCol®:

Drzewiecki,KathrynE.,etal.“CircularDichroismSpectroscopyofCollagenFibrillogenesis:ANewUseforanOldTechnique.”BiophysicalJournal,vol.111,no.11,2016,pp.2377–2386.,doi:10.1016/j.bpj.2016.10.023

Wingender,Brian,etal.“BiomimeticOrganizationofCollagenMatricestoTemplateBone-likeMicrostructures.”MatrixBIOLOGy,vol.52-54,2016,pp.384–396.,doi:10.1016/j.matbio.2016.02.004.

Burla,F.,Tauber,J.,Dussi,S.,Gucht,J.V.D.Koenderink,G.H.Stressmanagementincompositebiopolymernetworks.NaturePhysics15,549–553(2019).