Inidentifyingpeptidesfromproteinswithaknownsequence,itisoftenusefultobeabletopredicthowapeptidewillmigrateduringelectrophoresis,chromatography,ortwo-dimensionalpeptidemapping.

ThePepsortprogram,whichrunsasanadd-ontotheGCG/UWpackageofsequenceanalysisprograms,willcalculateelectrophoreticandchromatographicmobilitiesforpeptidesderivedfromasequencefile.ThisisaprogramwrittenbyattheSalkInstitutebyLisaCaballerousingdataofTonyHunter"s.Itisbynomeansfoolproof,butcanpredictthemobilitiesofsomepeptidesveryaccurately.

TorunPepsort,youneedtohavetheGCGsequenceanalysispackageonyourcomputer.

YoucanobtainPepsortfromtheSalkInstitutebyftpwithyourbrowserusingthislink

ftp://axp1.salk.edu/mobility/

Thisdirectorycontainsareadmefilethatexplainswhattodownloadandhowtoruntheprogram.

Partsofthereadmecanbefoundonthislinkedpage.

PepsortisusedinconjunctionwithaprogramcalledMobilitythatwillplotthepredictedmobilitiesofyourpeptides.Itisincludedinthesameftpdirectoryonaxp1.salk.edu.

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