• InVitroProductionofBovineEmbryos(P.J.HansenLab,Dept.ofAnimalSciences,UniversityofFlorida)Thisprotocoldescribeproceduresforinvitroproduction[IVP;i.e.invitromaturation(IVM),invitrofertilization(IVF),andinvitroculture(IVC)]ofembryos
• Mini-Chamberforregulatinggaseousenvironmentduringculture(P.J.HansenLab,Dept.ofAnimalSciences,UniversityofFlorida)Asimpleandinexpensivechamberforregulatinggaseousenvironmentofsmallcultureplates,suchasthoseusedforcultureofpreimplantationembryos,canbeconstructedusingdisposablemedia-filtrationdevisessuchasCorning’s115-mlsystem.Thefollowingisadescriptionofhowtomakesuchadevice
• Cellnumbercountinginpreimplantationembryos(P.J.HansenLab,Dept.ofAnimalSciences,UniversityofFlorida)Asimpleprocedurefordeterminingcellnumberinpreimplantationembryos.Whiletheprocedureiswrittenforfixedembryos,fixationisoptionalsincetheHoechst33342and4",6-diamidino-2-phenylindole,dihydrochloride(DAPI)penetratelivingcells.Thefixationstepisusefulwhenembryoswillbestoredforseveralweeksbeforedeterminationofcellnumber
• TUNELprocedureforpreimplantationembryos(P.J.HansenLab,Dept.ofAnimalSciences,UniversityofFlorida)AprotocolfordetectingcelldeathusingTUNEL.
• WholemountTUNELanalysisofXenopusembryos(PeterVize,XenopusMolecularMarkerResource)
• LineageAnalysisUsingRetrovirusVectors(CepkoLab,DepartmentofGenetics,Harusr/localdMedicalSchoolandHowardHughesMedicalInstitute,)Describetheuseofreplication-incompetentretroviralvectorsfortheanalysisoflinealrelationshipsindevelopingvertebratetissues.

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