Product Specification:

Alternative Name:	HSP86, Heat shock protein 90α
Clone:	K41009
Host:	Mouse
Isotype:	IgG2a
Immunogen:	Recombinant human Hsp90α.
UniProt ID:	P07900
Species reactivity:	Human, Mouse, Rat Rabbit
Recommended Dilutions/Conditions:	Flow Cytometry (1:100) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:	Detects a band of ~90kDa by Western blot.
Purity Detail:	Protein G-affinity purified.
Formulation:	Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
Handling:	Avoid freeze/thaw cycles. Protect from light.
Shipping:	Blue Ice Not Frozen
Long Term Storage:	+4°C
Scientific Background:	The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.

Enzo Life Sciences 自2007年始，Enzo Life Sciences先后合并ALEXIS Biochemicals、BIOMOL International等公司，为生命科学研究各个领域提供更全面的试剂产品。比如，肿瘤、细胞死亡、表观遗传、基因组学、免疫学、神经科学、信号转导及泛素和蛋白酶体等领域研究，都可以在Enzo Life Sciences中找到相应抗体、酶、蛋白、小分子抑制剂、产品文库及检测kit等产品。 产品评述样例 FluoForte被用在电生理研究中，来验证光遗传学可以作为控制精子受精和cAMP信号传导通路的有效工具。 拉春库林B被使用来研究G-肌动蛋白调节综合应激反应的机制。 类泛素化试剂盒被用来进行体外类泛素化修饰分析，来研究在DNA修复过程中 Mms21 SUMO连接酶的控制机制。 己糖激酶抗体被用在免疫印记实验中，来研究在DNA修复过程中 Mms21 SUMO连接酶的控制机制。 FK1抗体被用在免疫组化实验中，来研究电压控制的钙通道在自噬和维持神经元内稳态中的作用。 为了研究抗肿瘤反应中Dectin-1的作用，采用了Enzo Life Sciences公司的FK506，进行药物处理实验。 为了研究热激反应中eEF1A1在转录过程中的作用，采用了Enzo Life Sciences公司抗HSP27抗体，进行免疫印迹实验。 为了研究热激反应中eEF1A1在转录过程中的作用，采用了Enzo Life Sciences公司抗HSP70抗体，进行免疫印迹实验。 为了研究热激反应中eEF1A1在转录过程中的作用，采用了Enzo Life Sciences公司的抗HSF2抗体，进行EMSA实验。 为了研究热激反应中eEF1A1在转录过程中的作用，采用了Enzo Life Sciences公司的抗HSF1抗体，进行EMSA实验。 为了研究热激反应中eEF1A1在转录过程中的作用，采用了Enzo Life Sciences公司的p1730R载体，进行DNA克隆实验。 产品样例 Corticosterone ELISA kit Corticosterone ELISA kit HSP70/HSP72单克隆抗体(C92F3A-5) Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) HSP70/HSP72单克隆抗体(C92F3A-5) HSP70/HSP72单克隆抗体(C92F3A-5) Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) HO-1, 多克隆抗体 PGE2酶联免疫吸附试剂盒 Progesterone ELISA kit