Product Description

Sheep red blood cells coated with rabbit anti-sheep erythrocyte antiserum (hemolysin) have traditionally been used to assay the activity of the complement classical pathway in serum samples.These assays yield the CH50 titer of the serum (Morgan, B.P. (2000); Dodds, A.W. and Sim, R.B. (1997)).

EA are supplied at assay-ready concentrations in the traditional buffer used in CH50 assays (GVB⁺⁺).They are prepared fresh every Monday morning and need to be ordered by Friday in order to receive them the next week.They are shipped Monday afternoon by overnight courier for delivery on Tuesday (or Wednesday for most international shipments). They can usually be used for 2 weeks after preparation.They are shipped cold, but are not harmed by extended periods at room temperature (note that they circulate 60-90 days a 37^oC in vivo).They should be washed once before each use (3 min at 500 to 1000 x g at 4^oC) and resuspended at the original concentration in GVB⁺⁺ to reduce background.This procedure may also be used to concentrate the cells.This may be accomplished by reducing the volume used to resuspend the cells.

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Complement Technology的C1是级联反应中的第一个补体成分，称为补体经典途径。C1与抗原结合的抗体（免疫复合物）结合并被其激活，从而产生引发级联反应的蛋白酶。C1实际上是钙依赖性复合物中结合在一起的三种不同蛋白质（C1q，C1r和C1s）的非共价复合物。C1q通过其六个臂中的两个或多个与IgG或IgM的Fc结构域结合。多臂与免疫复合物的结合被认为会引入压力，从而导致复合物中的两个C1r蛋白（蛋白酶酶原）自身激活，从而产生两种活性的C1r丝氨酸蛋白酶（Morikis，D.和Lambris，JD（2005））。 。这些激活的C1r亚基裂解并激活复合物中的两个C1s蛋白酶酶原。活化的C1裂解补体成分C4，释放出C4a，并启动C4b与活化表面的共价连接。活化的C1也切割C2，并且C2的较大片段结合至表面附着的C4b，形成C4b，C2a，其为经典途径的C3 / C5转化酶。