Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pMKO.1
  (Search Vector Database)
• Backbone sizew/o insert(bp)6700
• Vector type
  Mammalian Expression, Retroviral, RNAi
• Selectable markers
  Puromycin

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  zfp423 shRNA
• gRNA/shRNA sequence
  CCCTGAATGTAACGTGAAGTT
• Species
  M. musculus (mouse)
• GenBank ID
  NM_033327
• Entrez Gene
  Zfp423(a.k.a. Ebfaz, Roaz, Zfp104, Znf423, ataxia1, mKIAA0760, nur12)

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteAgeI(destroyed during cloning)
• 3′ cloning siteEcoRI(not destroyed)
• 5′ sequencing primerpLXSN 5'
• 3′ sequencing primerpBABE-3
(Common Sequencing Primers)

Resource Information

• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry
• Articles Citing this Plasmid
  • 2 References

Depositor Comments