Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pAAV
  (Search Vector Database)
• Backbone sizew/o insert(bp)3812
• Vector type
  Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  NEB Stable
• Copy number
  High Copy

Gene/Insert1

• Gene/Insert name
  TRPV4
• Species
  R. norvegicus (rat)
• Entrez Gene
  Trpv4(a.k.a. Otrpc4, Vroac)
• PromoterCMV (after cre recombination)
• Tag/ Fusion Protein
  • p2A (C terminal on insert)

Cloning Informationfor Gene/Insert 1

• Cloning methodRestriction Enzyme
• 5′ cloning siteAscI(not destroyed)
• 3′ cloning siteKpnI(not destroyed)
• 5′ sequencing primerunknown
• 3′ sequencing primerF1ori-R
(Common Sequencing Primers)

Gene/Insert2

• Gene/Insert name
  ferritin (FTL and HTL1)
• Species
  H. sapiens (human)
• Entrez Gene
  FTL(a.k.a. LFTD, NBIA3)
• Entrez Gene
  FTH1(a.k.a. FHC, FTH, FTHL6, HFE5, PIG15, PLIF)
• Tag/ Fusion Protein
  • FLAG

Cloning Informationfor Gene/Insert 2

• Cloning methodUnknown
• 5′ sequencing primerunknown
• 3′ sequencing primerCMV-F
(Common Sequencing Primers)

Resource Information

• Supplemental Documents
  • pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA sequence
• Terms and Licenses
  • UBMTA
  • genOway Notice of RIghts
• Industry Terms
  • Not Available to Industry