Ordering

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Backbone

• Vector backbone
  pZE31
  (Search Vector Database)
• Backbone manufacturer
  Lutz and Bujard. 1997. Nucleic Acids Research,25: 1203-1210.
• Backbone sizew/o insert(bp)2000
• Total vector size (bp)5859
• Vector type
  Bacterial Expression, Synthetic Biology ; Optogenetics

Growth in Bacteria

• Bacterial Resistance(s)
  Chloramphenicol
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

Gene/Insert1

• Gene/Insert name
  ccaS (complement)
• Alt name
  green-light sensing histidine kinase
• Species
  Synechocystis sp. PCC6803
• Insert Size (bp)
  2259
• PromoterccaR
• Tag/ Fusion Protein
  • FLAG tag (C terminal on insert)

Cloning Informationfor Gene/Insert 1

• Cloning methodGibson Cloning
• 5′ sequencing primerGTTGTGCAACGTGCCAAC
• 3′ sequencing primerGTGAAAGTTGGAACCTCTTACG
(Common Sequencing Primers)

Gene/Insert2

• Gene/Insert name
  ccaR (complement)
• Alt name
  response regulator
• Species
  Synechocystis sp. PCC6803
• Insert Size (bp)
  702
• PromoterccaR
• Tag/ Fusion Protein
  • FLAG tag (C terminal on insert)

Cloning Informationfor Gene/Insert 2

• Cloning methodGibson Cloning
• 5′ sequencing primerGTGAAAAGTTCTTCTCCTTTACGC
(Common Sequencing Primers)

Gene/Insert3

• Gene/Insert name
  gfpmut3
• Alt name
  green fluorescent variant
• Species
  A. victoria
• Insert Size (bp)
  717
• PromotercpcG2Δ59

Cloning Informationfor Gene/Insert 3

• Cloning methodGibson Cloning
• 5′ sequencing primerGGCAATATCAACGGTGTAAAGC
• 3′ sequencing primerCTTTGAGTGAGCTGATACCG
(Common Sequencing Primers)

Resource Information

• Supplemental Documents
  • pSKA413 annotated GenBank file
• Terms and Licenses
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
• Industry Terms
  • Not Available to Industry