Ordering

This material is available to academics and nonprofits only.

Backbone

• Vector backbone
  pSico
  (Search Vector Database)
• Backbone manufacturer
  Jacks Lab
• Backbone sizew/o insert(bp)7566
• Total vector size (bp)7607
• Vector type
  Mammalian Expression, Lentiviral, RNAi, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s)
  Ampicillin
• Growth Temperature
  37°C
• Growth Strain(s)
  NEB Stable
• Copy number
  High Copy

Gene/Insert

• Gene/Insert name
  Raptor shRNA
• Alt name
  Rptor shRNA
• gRNA/shRNA sequence
  GCCCGAGTCTGTGAATGTAAT
• Species
  M. musculus (mouse)
• Entrez Gene
  Rptor(a.k.a. 4932417H02Rik, Rap, Raptor, mKIAA1303, r)
• PromoterU6

Cloning Information

• Cloning methodRestriction Enzyme
• 5′ cloning siteHpaI(destroyed during cloning)
• 3′ cloning siteXhoI(not destroyed)
• 5′ sequencing primerEGFP-C
(Common Sequencing Primers)

Resource Information

• A portion of this plasmid was derived from a plasmid made by
  pSico was obtained from Addgene (plasmid 11578). shRNA insert was synthesized independently but is based on a target sequence from the Broad RNAi Platform (TRCN0000077472).
• Terms and Licenses
  • UBMTA
• Industry Terms
  • Not Available to Industry

Depositor Comments