The HA-tag (YPYDVPDYA) antibody is immobilized to beaded agarose via amide linkages. The product could be utilized as an affinity matrix for rapid immunoprecipitation, isolation, purification and enrichment of recombinant proteins expressing the HA-tag.

Rabbit

10-20 mg/mL, antibody is covalently linked through amide bonds with NHS activated-SMCC then linked to thiolated agarose beads via thiol ether bonds.

1 mL beaded agarose suspended in 2 mL of glycerol

The antibody selectively captures peptides and proteins with HA-tag residues (YPYDVPDYA).

Approximately 0.1 µmole/mL or more than 5 mg/mL of 50kDa HA-tag proteins

IP, IAC for rapid isolation, purification snf enrichment of peptides or proteins with the HA-tag from cell lysate mixtures.

The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 of the human influenza hemagglutinin. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. Affinity chromatography is one of the most efficient and rapid methods to enrich and purify the HA-tag proteins.

Product is stable for at least 30 days at room temperature. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.