This antibody is obtained by immunization with purified human hemoglobin (Hb) and affinity purification with human Hb on agarose. The antibody could be utilized for detection andquantizationof the human hemoglobin using ELISA, WB gold colloid methods.

Goat

5 mg/mL in PBS or lyophilized powder.

Human Hemoglobin whole protein.

Purification

The antibody was affinity purified with pure human hemoglobin on agarose beads.

Specificity

The antibody recognizes human hemoglobin. It may cross-react with hemoglobin of other mammalian species.

ELISA (0.1-0.5 µg/mL);WB (0.25-1 µg/mL);IP; IAC;Gold colloid (to be optimized)

Hemoglobinis theiron-containingoxygen-transportmetalloproteinin thered blood cells.Hemoglobin concentration measurement is among the most commonly performedblood tests, usually as part of acomplete blood count.

Inmedicine,hemoglobinuriais a condition in which thehemoglobinis found in abnormally high concentrations in theurine. The condition is often associated withhemolytic anemia, in whichred blood cells(RBCs) are destroyed, thereby increasing levels of freeplasmahemoglobin. Excess hemoglobin is filtered by thekidneys, which excrete it into the urine, giving urine a purple color.

Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly.