The RealHelix™ 1-sec qRT-PCR kit [Probe] is designed to perform rapid real-time analysis of RNA samples using fluorescent probe based detection. This kit provides 2x premix and ROX reference dye. The convenient 2x concentrated premix contains an antibody-inhibited hot-start Taq polymerase (Ab+Taq polymerase), reverse transcriptase, RNase inhibitor, dNTPs, buffers, Mg²⁺, and stabilizing agent. The premix can also be used in combination with ROX reference dye in PCR instruments that are compatible with the evaluation of the ROX signal. The outstanding fast real-time assay within 40-60 min combined with high specificity and sensitivity is achieved with a unique buffer system and balanced enzyme mix.

Figure 1. Comparison of amplification plots of a general qRT-PCR enzyme (RealHelix™ qRT-PCR kit) and 1-sec qRT-PCR kit using either a standard cycling protocol or a fast cycling protocol. Multiplex Probe qRT-PCR reactions were done with the purified and mixed RNA of type A virus and type B virus (tenfold serial dilutions over range of 1 fg - 1 ng per reaction). Each 20μl reactions were operated on CFX96 real-time PCR detection system (BioRad).