HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0), the best choice to setup complex multiplexing PCR, is a pre-mixed solution containing HelixAmp™ Hot Taq DNA polymerase, dNTPs and optimized buffer as 2x concentration. HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0)is designed for hot-start PCR to allow the highest specificity and minimize the interruptions among the primers in a reaction mixture. This pre-mixed enzyme is highly suitable for up to 13-plex PCR, allele specific PCR (discriminating SNP), and applications for genotyping. The provided TuneUp™ solution helps to improve the yield and specificity in problematic PCRs. HelixAmp™ Ready-2x-MultiPlex (Ver. 2.0)provides the most suitable condition for efficient and reproducible multiplex or specific PCR.

Figure 1. Multiplex PCR of up to 8 targets using HelixAmp™ Ready-2x-MultiPlex. Multiplex PCR in single-tube reaction is performed with rice genomic DNA and mixture of allele-specific primer set corresponding to SNP. Primer set corresponding to each target was added into one-tube multiplex PCR one by one. M: Standard size marker 

Application

• Multiplex PCR 
• Allele specific PCR 

Contents

HelixAmp™ Ready-2x-MultiPlex PCR Master Mix (Ver. 2.0)containing HelixAmp™ Hot Taq DNA polymerase (Ver. 2.0), Buffer, dNTPs and stabilizing agents.

Quality Control

HelixAmp™ Ready-2x-Go series is evaluated by amplification compare with mixture of each component required in PCR for various size of target without further optimization at a different concentration of template DNA.