HelixAmp™ Hot-Taq polymerase (Ver. 2.0) is a chemically-modified form of purified Taq DNA polymerase and quite suitable for high-specific hot-start PCR, real-time PCR and multiplex PCR. The attached heat-labile chemical moiety makes Taq DNA polymerase inactive and suppresses the polymerization from non-specifically bound primers which occurs during the setting of PCR mix and first ramp-up of thermal cycling. During the first denaturation step of PCR the chemical moieties are released from Taq polymerase and the enzyme turns to be active. With the high specificity HelixAmp™ Hot-Taq polymerase (Ver.2.0)shows high performance in the reactions of genotyping (microsatellite or SNP), mutiplex PCR, and realtime PCR. For the maximum performance extremely pure dNTPs and TuneUp™ solutions are also included. TuneUp™ solution helps the DNA polymerase to efficiently amplify the problematic target region of high G+C content or structural problem.