Heat Labile UDG is a recombinant Uracil DNA glycosylase from marine bacterium BMTU 3346. UDG catalyzes the hydrolysis of the N-glycosidic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. Heat Labile UDG is fully active in the temperature range of 15 to 25℃ and inactivated at over 40℃. Due to this character, this enzyme is ideal for applying on the one-step RT-PCR system to remove the contaminated uracil-containing DNA in the reaction.

Application 

• Prevention of carryover contamination in RT-PCR

Enzyme activities: Hydrolyzes uracil-glycosidic bonds in single- and double-stranded DNA. 

Heat inactivation: +50°C for 2 minutes are sufficient for inactivation.  

Storage buffer: 20mM Tris-HCl(pH7.8), 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.05% Tween-20, 50% Glycerol

Purity: ≥90% (SDS-PAGE)

DNase contamination test: Not detectable (Incubation with 10U enzyme and pUC19 plasmid at 37°C, 1hr) 

Activity test: Removal of uracil-containing PCR product with one unit of enzyme

Stability: 24 months at -20°C