- Overview
- Data/Specifications
- Literature/Support
- How To Use
- Related Products
Overview
Type I collagen is the most abundant collagen and is found in connective tissues including tendon, ligament, dermis and blood vessel. It is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
Symbols/Related Terms:
- COL1A1
- COL1A2
- osteogenesis imperfecta
Data/Specifications
Reconstitution:Add 0.01-0.05 M acetic acid to yield 1-5 mg/mL collagen solution. Gentle mixing overnight at 4°C.
Purification: Partial pepsin digestion in acidic conditions and differential salt precipitation.
Source: mouse tail tendon
Form: lyophilized, 1 mg/vial
Purity:
Murine Collagen Type I at least 90%
Non- Collagen proteins below 2%
Storage: Collagen dissolved in acetic acid is stable at 4 °C for 1 month. Lyophilized collagen long term storage (2 years) at -20°C or lower.
Literature/Support
Mouse Type I Collagen Insert (PDF)
Note: Inserts are for review only. Please use the insert that was sent with the product to ensure that the correct revision insert is being used for the product purchased.
How To Use
Key Applicatons:
- Used for coating material for cell culture studies.
Note: may not be appropriate for 3D Gel preparation
