Klen Taq/OmniTaq 3/125微升(500 X 25微升rxns)/303

价格
¥6000.00
货号:303
浏览量:127
品牌:Klen Taq
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商品描述

OmniTaq 3 DNA polymerase is a mutant of Taq polymerase that makes the enzyme resistant to the inhibitory effects of blood, soil, and more.  It remains functional in up to 40% whole blood, especially in the presence of our enhancer products. OmniTaq 3 is suitable for direct amplification of samples containing plant tissues and feces. It also works in some concentrations of crude soil extract or inhibitory food matrices where other commercial enzymes fail. 

Vanderbilt Researchers detect SNP markers for antimalarial resistance using OmniTaq 3.

Catalog Number
303
MSDS
File
SDS- OmniTaq 3
Amount
125 ul (500 X 25 ul rxns)

Direct Blood PCR Performance of Inhibition-resistant OmniTaq 3

New Taq mutant dramatically outperforms the wild type Taqand remains functional in at least up to 40% blood.

A 1 kb target was amplified directly from human blood, present at 5-40% in 50 ul reaction, with 0.5 ul “1X” OmniTaq 3 or similar enzyme strength (1 μl/10U) of plain Taq (NEB). No PCR enhancer was used.  Control reactions (C) contained no blood but 10 ng human DNA.                                                             

Results: The new Taq mutant dramatically outperforms the wild type Taq  and remains functional in at least up to 40% blood. This enzyme strength overamplified and smeared the OT3 control.  Up to 5x less enzyme is needed for purified DNA (results not shown).          

Klen Taq的抑制性DNA聚合酶具有特别的选择,因为它们对广泛使用的强效PCR抑制剂具有很高的抵抗力,例如全血,血清,血浆,尿液,腐殖酸,胆汁盐,植物组织提取物,各种食品相关抑制剂,GITC,乙醇等。结果,这些酶可以在大多数商业Taq产品无法发挥作用的水平上耐受PCR中的此类抑制剂。Taq突变体的这一独特功能允许直接PCR各种临床,环境和法医原始样品,而无需进行DNA提取步骤。将这些产品与PCR增强剂鸡尾酒(PEC)配对可以进一步增强抑制性。