KEM-EN-TEC/碱性磷酸酶/10 mg、100 mg/4700

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货号:4700
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品牌:KEM-EN-TEC
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商品描述

Alkaline Phosphatase

Enzyme, Purified from calf intestine

ECO-TEK

Cat. No. 4700

Alkaline Phosphatase (AP) is purified from bovine intestinal mucosa by chromatography and filtration. AP is a zinc metaloenzyme for catalyzing the hydrolysis of an orthophosporic monoester to yield an alcohol and an orthophosphate. AP can be coupled to antibodies resulting in conjugates, used in ELISA, membrane & IHC applications, biosensors etc.

In ELISA, the main colorimetric substrate for AP is p-nitrophenyl phosphate (pNPP) which yields a yellow reaction product. Absorbance can be quantified by reading the OD at 405 nm. The kinetic reaction is terminated by the addition of a stop-solution.

This product is not for sale in the US.

Specifications

  • Available sizes:10 mg, 100 mg
  • Format:Ready-to-use (RTU)
  • Storage:2-8⁰C
  • Stability:3 years
  • Activity:> 1500 U/mg (4-nitrophenyl-phosphate, glycin as buffer)
  • Purity:More than 90% pure (chromatography)

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Instructions and Specifications
CoA
MSDS
Company Profile Brochure
Labels and Conjugates Brochure

Download your LOT specific Certificate of Analysis (CoA)

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ECO-TEK

  • Free of Toxic Preservatives
  • Free of Bovine Serum Albumin
  • Free of Harmful Organic Solvents – No NMP
  • Free of Hazardous Stop Solutions
All about eco-tek
Kementec缓冲液设计用于比色以及基于化学发光的测定,如ELISA,Western Blotting和IHC。提供的稳定而坚固的封闭缓冲液,稳定剂和稀释剂可以立即使用,并且大多数缓冲液都是ECO-TEK®:不含有毒的防腐剂免于牛血清白蛋白不含有害有机溶剂阻塞和稳定我们的产品组合包括用于传统的封闭缓冲ELISA和印迹,以及WellChampion ® - Kementec新一代平板封闭和保护缓冲区预涂层板。稀释剂和稳定剂Kementec提供了现成的样品稀释剂和稳定剂,可证明与辣根过氧化物酶(HRP)和碱性磷酸酶(AP)偶联的蛋白质具有出色的稳定性。这些稳定剂可防止酶活性损失并保持蛋白质分子构象。