ProductDescription
Datahasshowndirecttransdifferentiationbetweendifferentgermlayerswhentransfectedwithacocktailofdefinedtranscriptionfactorgenes1,3.Asupplementofthissmallmoleculecocktailincellculturemediasimplifiestheprocessandincreasestheproductivityofneuralandneuronalcellgeneration.
Repro-nLSCisacocktailofthreesmallmoleculeinhibitors.ItincludesLDN193189,SB431542andCHIR99021.UsingthecombinationofsynergisticSMADinhibitionthroughBMPandTGF-betapathwaystogetherwithspecificGSK-3betainhibition,arecentstudyhaspresentedthatpostnatalhumanfibroblasts(HPF),transducedwithAscl1andNgn2,efficientlyconvertedintofunctionalneuron-likecellswithyieldsupto>200%andneuronalpuritiesupto>80%.Inadditiontotheconversionofmesenchymalcellstoinducedneurons(iNs),thismethodhasalsobeentestedintheconversionofothersomaticcelltypes(eg.Cordblood-derivedstemcells)toiNs3.
Technicalinformation:
| CocktailFormulation: 1000xDMSOstocksolution | 0.5mMofLDN193189(BMPinhibitor), 10mMofSB431542(TGF-ß1inhibitor),and 2mMofCHIR99021(GSK3ßinhibitor) | |
| Purity: | >98%(foreachcompound) |
ShippingCondition:Theproductisshippedinaglassvialatambienttemperature.
Storage:StoreDMSOsolutionat-20oC.
Usage:DiluteRepro-nLSCstocksolution(1000x)at1:1000intoyourreprogrammingmediatoinitiateneuronalconversion.
Reference:
| 1. | VierbuchenT,etal.Directconversionoffibroblaststofunctionalneuronsbydefinedfactors.Nature.2010.463(7284):1035-41. |
| 2. | ChambersSM,etal.HighlyefficientneuralconversionofhumanESandiPScellsbydualinhibitionofSMADsignaling.NatBiotechnol.200927(3):275-80. |
| 3. | LadewigJ.,etal.Smallmoleculesenablehighlyefficientneuronalconversionofhumanfibroblasts.NatMethods.2012.9(6):575-8. |
Cellagen的FlexiWash FW400自动膜/凝胶洗涤站设计用于自动Western blot膜洗涤和孵育过程。将蛋白质转移到膜上后，将膜加载到FlexiWash上以进行自动封闭，孵育和洗涤循环。用户只需要准备所需的试剂，启动印迹程序，然后让FW400进行其余操作即可。凭借独特的液体处理设计，FW400同时支持四个清洗/孵育过程，每个过程均可独立编程，并针对特定应用实现全自动。FW400也可用于蛋白质或DNA凝胶染色和脱色，或涉及重复液体处理和孵育周期的任何其他应用。
